G9136

Porcine Gelatin

from porcine skin, Type A, powder, γ-irradiated, suitable for cell culture, BioXtra

• CAS Number: 9000-70-8
• EC Number: 232-554-6
• MDL number: MFCD00081638
• UNSPSC Code: 12352202
• NACRES: NA.75

Properties

• product name: Gelatin from porcine skin, Type A, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture
• biological source: Porcine skin
• Quality Level: 200
• sterility: γ-irradiated
• type: Type A
• product line: BioXtra
• form: lyophilized powder
• packaging: pkg of 10 mg
• technique(s): cell culture | mammalian: suitable
• surface coverage: 100‑200 μg/cm²
• gel strength: ~300 g Bloom
• solubility: soluble 20 mg/mL, clear, colorless (in warm tissue culture medium)
• shipped in: ambient
• storage temp.: room temp

Description

Application

This product is recommended for use as a cell culture substratum at 100-200 μg/cm². The optimal concentration does depend on cell type as well as the application and research objectives.

Gelatin has been used in many applications. It has use in coating cell culture to improve attachment of cells, being added to PCR to stabilize Taq DNA, as a blocking reagent in Western blotting, ELISA, and immunochemistry, and as a component of media for species differentiation in bacteriology. As a biocompatible polymer, it has used as a delivery vehicle for release of active biomolecules and in generation of scaffolds for tissue engineering applications. In the pharmaceutical industry, geltan can be used as a suspending and encapsulating agent, among other applications.

Components

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. Proteins are extracted by boiling the relevant skin, tendons, ligaments, bones, etc. in water. Type A gelatin is derived from acid-cured tissue. Type B is derived from lime-cured tissue.

Caution

Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.

Preparation Note

This product is derived from porcine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentration. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g.

This product is a type A lyophilized powder, suitable for cell culture, with a gel strength of 300.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves