69182

DNase I, RNase free

For applications in which maintenance of RNA integrity is critical

• Synonym(s): RNase-free DNase I
• UNSPSC Code: 12352202
• NACRES: NA.85

Properties

• form: liquid
• manufacturer/tradename: Novagen^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• technique(s): DNA extraction: suitable
• suitability: suitable for nucleic acid purification
• application(s): diagnostic assay manufacturing
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

RNase-free DNase I digests either single- or double-stranded DNA, producing a mixture of mono- and oligonucleotides. Purified to be free of RNase, this preparation is qualified for applications in which maintenance of the integrity of RNA is critical. The enzyme selectively degrades DNA in the presence of RNA and can be used to remove DNA template following in vitro transcription reactions. This enzyme is also useful in other applications such as DNase footprinting and nick translation.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit will degrade 1 μg DNA in 10 minutes at 37°C. The reaction mixture (50 µl) contains 80 mM HEPES pH 7.5, 10 mM NaCl, 5 mM MgCl₂, 10 mM DTT, 1 µg plasmid DNA, and enzyme.

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable