06-720
Anti-HDAC1 Antibody
Upstate®, from rabbit
Synonym(s):
histone deacetylase 1, reduced potassium dependency, yeast homolog-like 1
About This Item
Recommended Products
biological source
rabbit
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat, human, mouse
packaging
antibody small pack of 25 μg
manufacturer/tradename
Upstate®
technique(s)
activity assay: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... HDAC1(3065)
mouse ... Hdac1(433759)
rat ... Hdac1(297893)
General description
Specificity
Immunogen
Application
5 μg of a previous lot immunoprecipitated HDAC1 from 500 μg of 3T3/A31 RIPA lysate.
Immunocytochemistry:
This antibody has been reported to immunostain HDAC1 in 1% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized 3T3 cells.
HDAC Activity:
This antibody has been reported to immunoprecipitate active HDAC.
Epigenetics & Nuclear Function
Histones
Quality
Western Blot Analysis:
0.5-2 μg/mL of this lot detected HDAC1 in RIPA lysates from 3T3/A31 cells.
Target description
Physical form
Storage and Stability
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Other Notes
Legal Information
Disclaimer
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recommended
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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