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  • [Platinum tetrachloride coupled PEG-PEI as drug carrier and gene delivery vector].

[Platinum tetrachloride coupled PEG-PEI as drug carrier and gene delivery vector].

Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences (2009-03-03)
Lu Zhao, Huan Chen, Dan Chen, Yi-Ping Hu, Hai Yu, Qing-Qing Wang, Gu-Ping Tang, Xiu-Rong Hu
ABSTRACT

To construct a drug carrier and gene vector PEG-PEI-Pt. Polyethyleneglycol (PEG) was coupled to polyethylenimine (PEI 600) and platinum tetrachloride; PEG-PEI-Pt complex was formed in ethanol. The complex was characterized by XRD, UV-VIS and FT-IR and the DNA condensation was tested by electrophoretic mobility shift assay. The cell viability was evaluated by MTT assay in Hela, B16, A293 and COS-7 cells and in vitro transfection efficiency was measured in A293 and B16 cells. The structure of PEG-PEI-Pt was characterized by XRD, UV-VIS and FT-IR. PEG-PEI-Pt complex was able to bind DNA at N/P weight ratio of 0.4:1; the complex showed cytotoxicity on Hela and B16 cells. The complex had higher transfection efficiency in A293 and B16 cells than PEI 600. A novel drug carrier and gene vector PEG-PEI-Pt was constructed successfully.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Platinum(IV) chloride, ≥99.99% trace metals basis
Sigma-Aldrich
Platinum(IV) chloride, 96%
Sigma-Aldrich
Platinum(IV) chloride, ≥99.9% trace metals basis