CP74
Anti-Vinculin Mouse mAb (V284 or VLN01)
liquid, Calbiochem®, from mouse
Synonym(s):
Anti-Vinculin antibody
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
V284, monoclonal
VLN01, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
isotype
IgG1
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... VCL(7414)
Related Categories
General description
Protein G purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with mouse myeloma SP2/0 cells. Recognizes the ~130 (vinculin) and ~150 kDa (meta-vinculin) proteins.
Recognizes the ~130 (vinculin) and ~150 kDa (meta-vinculin) proteins in skin tissue.
This Anti-Vinculin Mouse mAb (V284 or VLN01) is validated for use in Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of Vinculin.
Immunogen
Human
semi-purified, human vinculin
Application
Immunoblotting (1 g/ml, chemiluminescence)
Immunoprecipitation (2 g/ml lysate)
Paraffin Sections (4-8 g/ml, heat pre-treatment required)
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Physical form
In 10 mM PBS, 0.2% BSA, pH 7.4.
Analysis Note
Negative Control
Normal mouse serum
Normal mouse serum
Positive Control
Skin cells or tissue
Skin cells or tissue
Other Notes
Palovuori, R., and Eskelinen, S. 2000 Eur. J. Cell Biol.79, 961.
Bakolitsa, C., et al. 1999 Cell99, 603.
Rudiger, M., et al. 1998 Bioessays20, 733
Tidball, J.G., et al. 1999 J. Biol. Chem.274, 33155
Goldmann, W.H., et al. 1996 J. Muscle Res. Cell Motil.17, 1.
Asijee, G.M., et al. 1990 Eur. J. Biochemistry189, 131
Otto, J.J. 1990 Cell Motil. Cytoskeleton16, 1.
Bakolitsa, C., et al. 1999 Cell99, 603.
Rudiger, M., et al. 1998 Bioessays20, 733
Tidball, J.G., et al. 1999 J. Biol. Chem.274, 33155
Goldmann, W.H., et al. 1996 J. Muscle Res. Cell Motil.17, 1.
Asijee, G.M., et al. 1990 Eur. J. Biochemistry189, 131
Otto, J.J. 1990 Cell Motil. Cytoskeleton16, 1.
This antibody is useful for studies of the role of vinculin in platelet activation. Vinculin (Ab-1) exhibits membrane and cytoplasmic staining when used in immunohistochemistry. For staining of formalin-fixed paraffin sections, boil in 10 mM citrate buffer, pH 6.0, for 10-20 min, followed by cooling at room temperature for 20 min. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Not finding the right product?
Try our Product Selector Tool.
recommended
Product No.
Description
Pricing
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Christian Redeker et al.
PloS one, 8(10), e79050-e79050 (2013-10-30)
The Notch signaling pathway mediates the direct communication between adjacent cells and regulates multiple developmental processes. Interaction of the Notch receptor with its ligands induces the liberation of the intracellular portion of Notch (NICD) referred to as regulated intramembraneous proteolysis
Synergistic apoptotic effects in cancer cells by the combination of CLK and Bcl-2 family inhibitors.
Aiko Murai et al.
PloS one, 15(10), e0240718-e0240718 (2020-10-17)
Emerging evidence indicates that alternative splicing plays a critical role in cancer progression through abnormal expression or mutation of splicing factors. Small-molecule splicing modulators have recently attracted considerable attention as a novel class of cancer therapeutics. CDC-like kinases (CLKs) are
Job de Lange et al.
American journal of cancer research, 2(5), 492-507 (2012-09-08)
The p53 tumor suppressor pathway is inactivated in cancer either via direct mutation or via deregulation of upstream regulators or downstream effectors. P53 mutations are rare in uveal melanoma. Here we investigated the role of the p53 inhibitor Hdmx in
A Freije et al.
Oncogene, 31(50), 5180-5192 (2012-02-22)
Human epidermis is continuously exposed to environmental mutagenic hazard and is the most frequent target of human cancer. How the epidermis coordinates proliferation with differentiation to maintain homeostasis, even in hyperproliferative conditions, is unclear. For instance, overactivation of the proto-oncogene
Mizuko Ikeda et al.
Critical care (London, England), 19, 332-332 (2015-09-19)
There is a sex difference in the risk of ischemic acute kidney injury (AKI), and estrogen mediates the protective effect of female sex. We previously demonstrated that preprocedural chronic restoration of physiologic estrogen to ovariectomized female mice ameliorated AKI after
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service