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  • Iodinated soluble adult worm protein preparations of Schistosoma mansoni do not induce immediate hypersensitivity reactions but retain other immunogenic properties.

Iodinated soluble adult worm protein preparations of Schistosoma mansoni do not induce immediate hypersensitivity reactions but retain other immunogenic properties.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas (1991-01-01)
R M Passos, L H Pereira, C O Chavez, M Chamone, I F Heneine
ABSTRACT

Samples of Schistosoma mansoni soluble adult worm proteins (SWAP) were iodinated with 4-15 mumol I/mg protein using iodine monochloride. The capacity to elicit immediate hypersensitivity reactions of the iodinated derivatives was compared to that of the native SWAP preparations. The degranulation of mast cells from infected mice decreased with increasing iodine incorporation and was absent in fully iodinated samples containing 15 mumol I/mg protein. The response of guinea pigs and humans to the intradermal test with iodinated SWAP also decreased in proportion to iodine incorporation, and no responses were obtained with fully iodinated samples. No false-positive tests were observed. Antibodies to the fully iodinated extracts generated in C57BL/10 mice reacted by ELISA with the unmodified proteins and by immunoprecipitation on agar gel. The immunoprecipitation pattern suggested that some epitopes were altered by iodination.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Iodine monochloride solution, 1 M in acetic acid
Sigma-Aldrich
Wijs (iodine monochloride) solution | Chloroiodide | 7790-99-0 solution
Sigma-Aldrich
Iodine monochloride, 99.998% trace metals basis
Sigma-Aldrich
Iodine monochloride, reagent grade, ≥95%
Sigma-Aldrich
Iodine monochloride solution, 1.0 M in methylene chloride
Sigma-Aldrich
Iodine monochloride, ACS reagent, 1.10±0.1 I/Cl ratio basis