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Key Documents

MAB2053

Sigma-Aldrich

Anti-Disialoganglioside GD3 Antibody, clone MB3.6

clone MB3.6, Chemicon®, from mouse

Synonym(s):

GD3 Antibody

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

MB3.6, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable

isotype

IgG3

shipped in

wet ice

target post-translational modification

unmodified

Related Categories

Specificity

Reacts specifically with human GD3 ganglioside (Cheresh et al., 1984). No cross-reactivity with other gangliosides purified from neuroblastoma or melanoma cells.

Immunogen

FM9 melanoma cells

Application

Anti-Disialoganglioside GD3 Antibody, clone MB3.6 is an antibody against Disialoganglioside GD3 for use in FC, IF, IH.
Immunohistochemistry (frozen tissue sections)

Immunofluorescence of cells in culture

Flow cytometry: 1-2 μg per 10E6 cells

Lysis of GD3 positive (melanoma) cells in presence of complement (Cheresh et al., 1985).

Induction of antibody dependent cellular cyotoxicity (ADCC) {Ortaldo JR et al 1987 J Immunol 138(10):3566-3572.

TLC analysis: Sjoberg, ER (1995) J. Biol. Chem. 270(7):2921-2930.{http://www.jbc.org/cgi/content/full/270/7/2921}





Optimal working dilutions must be determined by end user.

Physical form

Format: Purified
Liquid in 0.02M PB pH 7.6, 0.25M NaCl containing 0.1% NaN3.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Rebecca L Mather et al.
Scientific reports, 9(1), 8609-8609 (2019-06-15)
Medulloblastoma (MB), the most common malignant paediatric brain tumour occurs in the cerebellum. Advances in molecular genomics have led to the identification of defined subgroups which are associated with distinct clinical prognoses. Despite this classification, standard therapies for all subgroups
Suzanne M Birks et al.
Neuro-oncology, 13(9), 950-960 (2011-08-03)
The expression of ganglioside GD3, which plays crucial roles in normal brain development, decreases in adults but is upregulated in neoplastic cells, where it regulates tumor invasion and survival. Normally a buildup of GD3 induces apoptosis, but this does not
Seth H Pincus et al.
Vaccine, 30(32), 4849-4858 (2012-05-29)
Group B streptococcus (GBS) is a major cause of neonatal sepsis and meningitis. Despite aggressive campaigns using antenatal prophylactic antibiotic therapy, infections continue. Developing an effective maternal vaccine is a public health priority. Antibody (Ab) to the capsular polysaccharide (CPS)
Effie E Bastounis et al.
Scientific reports, 9(1), 18209-18209 (2019-12-05)
Endothelial cells respond to changes in subendothelial stiffness by altering their migration and mechanics, but whether those responses are due to transcriptional reprogramming remains largely unknown. We measured traction force generation and also performed gene expression profiling for two endothelial

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