Ageing and retail display time in raw beef odour according to the degree of lipid oxidation.

Ageing and retail display time in raw beef odour according to the degree of lipid oxidation.

Food chemistry (2017-10-19)

Virginia C Resconi, Mónica Bueno, Ana Escudero, Danielle Magalhaes, Vicente Ferreira, M Mar Campo

ABSTRACT

This study aims to assess the changes in beef aroma over time when steaks from pre-aged knuckles are stored in retail display under high oxygen conditions for 15 or 22days in vacuum conditions. Odorous volatile compounds were analysed by solid-phase microextraction/gas chromatography-mass spectrometry. Results were grouped as low, medium and high oxidative groups according to thiobarbituric acid reactive substances values after 9days of display. The intensity of off-odours in the raw meat increased with ageing and display time and oxidative groups. Based on correlations between the variables and regressions of the compounds through display, eight compounds were proposed as odour shelf-life markers. Among them, five were most sensitive and precise in all oxidative groups: 1-hexanol in meat aged for 15days, ethyl- octanoate and 2-pentylfuran in meat aged for 22days, and pentanoic and hexanoic acids in the two studied ageing times.

MATERIALS

Product Number

Brand

Product Description

57902-U

Supelco

SPME Fiber Assembly Polydimethylsiloxane/Divinylbenzene (PDMS/DVB), d_f 65 μm(PDMS/DVB, for use with autosampler, needle size 23 ga, metal alloy fiber

57550-U

Supelco

SPME StableFlex^™ fiber assortment kit, for use with manual holder

57321-U

Supelco

SPME fiber assortment kit 2, for use with autosampler, size 24 ga

57324-U

Supelco

SPME fiber assortment kit 4, for use with manual holder, size 24 ga

57286-U

Supelco

SPME fiber assortment kit 2, for use with autosampler, needle size 23 ga

57551-U

Supelco

SPME StableFlex^™ fiber assortment kit, needle size 24 ga, for use with autosampler

57285-U

Supelco

SPME fiber assortment kit 1, for use with autosampler, needle size 23 ga

57287-U

Supelco

SPME fiber assortment kit 4, for use with autosampler, needle size 23 ga

57284-U

Supelco

SPME StableFlex^™ fiber assortment kit, needle size 23 ga, StableFlex fiber, for use with autosampler

57311

Supelco

SPME Fiber Assembly Polydimethylsiloxane/Divinylbenzene (PDMS/DVB), d_f 65 μm(PDMS/DVB, needle size 24 ga, for use with autosampler

57320-U

Supelco

SPME fiber assortment kit 2, for use with manual holder, needle size 24 ga

57325-U

Supelco

SPME fiber assortment kit 4, for use with autosampler, size 24 ga

57306

Supelco

SPME fiber assortment kit 1, for use with manual holder, needle size 24 ga

57307

Supelco

SPME fiber assortment kit 1, for use with autosampler, needle size 24 ga

108383

Sigma-Aldrich

1,1,3,3-Tetramethoxypropane, 99%

115606

Sigma-Aldrich

Hexanal, 98%

255653

Sigma-Aldrich

trans-2-Nonenal, 97%

269956

Sigma-Aldrich

trans-2-Octenal, technical grade, 94%

W316504

Sigma-Aldrich

trans-2-Heptenal, ≥95%, stabilized, FG

W342300

Sigma-Aldrich

trans-2-Undecenal, ≥95%, stabilized, FG

57362-U

Supelco

SPME fiber assortment kit 5, needle size 23 ga, for use with autosampler

W331708

Sigma-Aldrich

2-Pentylfuran, ≥98%, FG

148962

Sigma-Aldrich

Ethyl hexanoate, ≥99%

W287407

Sigma-Aldrich

Phenylacetaldehyde, ≥95%, FCC, FG

57294-U

Supelco

SPME fiber assembly polyacrylate (PA), d_f 85 μm(PA, for use with autosampler, needle size 23 ga

D215554

Sigma-Aldrich

Dipropylene glycol, 99%, mixture of isomers

57906-U

Supelco

SPME fiber assembly Carboxen/Polydimethylsiloxane (CAR/PDMS), d_f 85 μm(CAR/PDMS, for use with autosampler, needle size 23 ga, metal alloy fiber

57912-U

Supelco

SPME Fiber Assembly, 50/30µm DVB/CAR/PDMS, Metal Alloy (1cm), needle size 23 ga, Autosampler, pk of 1 ×, plain gray hub

57302

Supelco

SPME fiber assembly Polydimethylsiloxane (PDMS), d_f 7 μm(PDMS, needle size 24 ga, for use with manual holder

57327-U

Supelco

SPME Fiber Assembly Polydimethylsiloxane/Divinylbenzene (PDMS/DVB), d_f 65 μm(PDMS/DVB, needle size 24 ga, StableFlex, for use with autosampler