Application of the Fenton process in the elimination of helminth eggs.

Application of the Fenton process in the elimination of helminth eggs.

Journal of water and health (2014-12-05)

Sandra Isabel Escobar-Megchún, Hugo Alejandro Nájera-Aguilar, Magaly González-Hilerio, Javier Gutiérrez-Jiménez, Rubén Fernando Gutiérrez-Hernández, María Neftalí Rojas-Valencia

PMID25473981

ABSTRACT

This study relates to a method for evaluating the degradation efficiency of Ascaris lumbricoides eggs through a Fenton reaction, using hydrogen peroxide (H2O2) at 100, 250, and 500 mg/L, ferrous sulfate (FeSO4) at 458 mg/L Fe(2+) and pH values of 3, 4.5, and 6. The experiments were conducted according to a 3(2) experimental design, with 1:1, 3:1, and 5:1 (H2O2/Fe(2+)) molar ratios. The oxidation and flocculation stages were performed at 130 rpm during 2 hours and at 25-30 rpm during 20 min, respectively. As a result of the Fenton reaction, an average of 91.2% Ascaris lumbricoides egg degradation was achieved at pH 6 using a 500 mg/L dose of H2O2 and a 3:1 (H2O2/Fe(2+)) molar ratio. Thus, this process is an alternative for eliminating parasites that are resistant to conventional disinfection processes and significantly reduces the human health hazard they represent.

MATERIALS

Product Number

Brand

Product Description

209309

Sigma-Aldrich

Iron, powder, −325 mesh, 97%

IRMM524B

Iron, IRMM^®, certified reference material, 0.5 mm wire

88597

Millipore

Hydrogen peroxide solution, 3%, suitable for microbiology

267945

Sigma-Aldrich

Iron, chips, 99.98% trace metals basis

356808

Sigma-Aldrich

Iron, foil, thickness 0.1 mm, ≥99.9% trace metals basis

F8263

Sigma-Aldrich

Iron(II) sulfate heptahydrate, suitable for plant cell culture, ≥99%

F8633

Sigma-Aldrich

Iron(II) sulfate heptahydrate, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99%

44890

Sigma-Aldrich

Iron, puriss. p.a., carbonyl-Iron powder, low in magnesium and manganese compounds, ≥99.5% (RT)

GF62438520

Iron, rod, 200mm, diameter 25mm, as drawn, 98+%

GF90625181

Iron, rod, 1000mm, diameter 2.0mm, as drawn, 99.95%

GF01160143

Iron, foil, 100x100mm, thickness 2.0mm, as rolled, 99.95%

GF60615503

Iron, rod, 100mm, diameter 2.0mm, as drawn, 99.95%

GF45140925

Iron, rod, 50mm, diameter 5.0mm, as drawn, 99.99+%

GF60679862

Iron, rod, 500mm, diameter 6.35mm, as drawn, 98+%

GF16200032

Iron, rod, 100mm, diameter 10.0mm, as drawn, 99.99+%

GF08067214

Iron, foil, 100x100mm, thickness 2.0mm, as rolled, armco« soft ingot 99.8%

GF42616076

Iron, rod, 100mm, diameter 5.0mm, as drawn, 99.99+%

GF30262812

Iron, rod, 500mm, diameter 25mm, as drawn, 98+%

GF45684232

Iron, rod, 25mm, diameter 10.0mm, as drawn, 99.99+%

GF15174255

Iron, tube, 200mm, outside diameter 10.6mm, inside diameter 10.0mm, wall thickness 0.3mm, as drawn, 99.8+%

GF88927543

Iron, rod, 1000mm, diameter 2.0mm, as drawn, 99.99+%

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Iron, foil, not light tested, 25x25mm, thickness 0.002mm, 99.85%

GF26423737

Iron, foil, 25x25mm, thickness 0.9mm, as rolled, 99.5%

GF48235013

Iron, tube, 1000mm, outside diameter 2.0mm, inside diameter 1.6mm, wall thickness 0.2mm, as drawn, 99.5%

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Iron, tube, 100mm, outside diameter 12.7mm, inside diameter 9.5mm, wall thickness 1.6mm, annealed, 99.5%

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Iron, foil, not light tested, 300x300mm, thickness 0.05mm, hard, 99.5%

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Iron, tube, 200mm, outside diameter 25.0mm, inside diameter 21mm, wall thickness 2mm, annealed, 99.5%

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Iron, tube, 1000mm, outside diameter 8.0mm, inside diameter 5mm, wall thickness 1.5mm, annealed, 99.5%

GF67037766

Iron, rod, 100mm, diameter 50mm, as drawn, 98+%