- New insights into ubiquitin E3 ligase mechanism.
New insights into ubiquitin E3 ligase mechanism.
Nature structural & molecular biology (2014-04-05)
Christopher E Berndsen, Cynthia Wolberger
PMID24699078
ABSTRACT
E3 ligases carry out the final step in the ubiquitination cascade, catalyzing transfer of ubiquitin from an E2 enzyme to form a covalent bond with a substrate lysine. Three distinct classes of E3 ligases have been identified that stimulate transfer of ubiquitin and ubiquitin-like proteins through either a direct or an indirect mechanism. Only recently have the catalytic mechanisms of E3 ligases begun to be elucidated.
MATERIALS
Product Number
Brand
Product Description
Lysine hydrochloride, European Pharmacopoeia (EP) Reference Standard
Lysine acetate, European Pharmacopoeia (EP) Reference Standard
Supelco
L-Lysine monohydrochloride, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
Supelco
L-Lysine monohydrochloride, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
L-Lysine monohydrochloride, from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, 98.5-101.0%