The supplier advises that they have not yet validated any lysis buffers for use with cell samples in conjunction with this kit. Additionally, they recommend using an untreated, clear, flat-bottom plate for colorimetric measurements. Therefore, it would be preferable to transfer the samples to such a plate for running the assay.
MAK317
Sorbitol Dehydrogenase Assay Kit
sufficient for 100 colorimetric tests
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1 KIT
$741.00
About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
detection method
colorimetric
relevant disease(s)
endocrinological disorders, diabetes; gastrointestinal diseases
storage temp.
−20°C
Related Categories
General description
Sorbitol Dehydrogenase (SDH) is an enzyme that catalyzes the interconversion of sorbitol and fructose. Elevated blood serum SDH levels indicate liver damage. Thus, SDH plays an important role in the diagnosis of liver disease, especially in combination with aminotransferases. SDH levels are also measured to evaluate diabetic complications such as proliferative diabetic retinopathy.[1][2]
Sorbitol Dehydrogenase (SDH) is an enzyme that catalyzes the interconversion of sorbitol and fructose. Elevated blood serum SDH levels indicate liver damage; thus, SDH plays an important role in the diagnosis of liver disease, especially in combination with aminotransferases. SDH levels are also measured to evaluate diabetic complications such as proliferative diabetic retinopathy.
Features and Benefits
Compatible with high-throughput handling systems.
Suitability
Suitable for determining sorbitol dehydrogenase (SDH) activity in a number of biological samples such as plasma, serum, urine, tissue, and culture media.
Principle
Sorbitol dehydrogenase assay is a non-radioactive, colorimetric assay. It is based on the reduction of the tetrazolium salt MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium bromide) in a NADH-coupled enzymatic reaction. The reduced form of MTT exhibits an absorption maximum at 565 nm and the increase in absorbance at 565 nm is directly proportional to the enzyme activity. This assay is based on a kinetic reaction. Sorbitol dehydrogenase (SDH) activity can be determined in a number of biological samples (e.g., plasma, serum, urine, tissue, and culture media).
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
3 - Flammable liquids
Flash Point(F)
75.2 °F - closed cup
Flash Point(C)
24 °C - closed cup
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What are the recommended methods for homogenizing or lysing cells in a 96-well plate for the MAK317 Sorbitol Dehydrogenase assay kit? The manual advises against using proteolytic enzymes and suggests using a rubber policeman, which is not suitable for a 96-well plate. Are there any recommendations for a mild lysing buffer with minimal impact on the assay outcome? Additionally, is it possible and recommended to run the assay in the same 96-well plate, or is transferring the samples to a new plate necessary?
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