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C-41000

Sigma-Aldrich

Trypsin / EDTA

0.04% Trypsin / 0.03% EDTA, 30 ml

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.78

form

aqueous solution

packaging

pkg of 30 ml

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−20°C

General description

Trypsin / EDTA (0.04% / 0.03%)

Application

Trypsin is still the most widely employed enzyme used for cell detachment. PromoCell´s Trypsin Solutions exhibit standardized enzyme activity and contain trypsin from extra-pure lots. This Trypsin/EDTA Solution has a ratio of 0.04% trypsin and 0.03% EDTA and is available in different sizes.

Quality

All lots of PromoCell Cell Detachment Reagents are subjected to comprehensive quality control tests. Each lot is routinely tested for biological function, absence of cytotoxicity, and physical parameters such as osmolality and pH level. Approved in-house lots are used as a reference. In addition, all lots have been tested for the absence of microbial contaminants (fungi, bacteria).

Storage and Stability

Store at -20°C in the dark immediately after arrival. Refrigerate thawed solution at 4 to 8°C. If stored properly, the product is stable until the expiry date stated on the label.

Subculture Routine

Click here for more information.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Cell systems, 11(5), 509-522 (2020-10-11)
The need to derive and culture diverse cell or tissue types in vitro has prompted investigations on how changes in culture conditions affect cell states. However, the identification of the optimal conditions (e.g., signaling molecules and growth factors) required to maintain

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