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Sigma-Aldrich

Anti-c-Jun/AP-1 (Ab-3) Mouse mAb (76)

liquid, clone 76, Calbiochem®

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About This Item

UNSPSC Code:
12352203

biological source

mouse

Quality Level

antibody product type

primary antibodies

clone

76, monoclonal

form

liquid

does not contain

preservative

species reactivity

human, mouse, rat

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

isotype

IgG1

shipped in

wet ice

storage temp.

−20°C

General description

Purified mouse monoclonal antibody. Recognizes the ~32-39 kDa c-Jun/AP-1 protein.
Recognizes the ~39 kDa c-Jun/AP-1 protein in proliferating cells and most tumor tissues.

Immunogen

recombinant jun protein

Application


Immunoblotting (2.5 g/ml)
Paraffin Sections (2.5 g/ml, pressure cooker pre-treatment required)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 0.05 M sodium phosphate buffer, 50% glycerol.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Negative Control
Quiescent cells
Positive Control
Growing cells (such as HeLa) or breast or colon tissue

Other Notes

This is the preferred c-Jun antibody for immunoblotting. Antibody should be titrated for optimal results in individual systems.
Volm, M., et al. 1993. Clin. Exp. Metastasis11, 325.
Angel, P., et al. 1988. Nature332, 166.
Rouscher III, F.J., et al. 1988. Science240, 1010.
Sassone-Corsi, P., et al. 1988. Cell54, 553.
Bohmann, D., et al. 1987. .Science238, 1386.
Vogt, P.K., et al. 1987. Proc. Natl. Acad. Sci. USA84, 3316.
Arndt, K., et al. 1986. Proc. Natl. Acad. Sci. USA83, 8516.
Cavaleri, F., et al. 1985. Virology143, 680.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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