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MABN24

Sigma-Aldrich

Anti-pan-Shank Antibody, clone N23B/49

clone N23B/49, from mouse

Synonym(s):

SH3 and multiple ankyrin repeat domains 2, SH3 and multiple ankyrin repeat domains protein 2, proline-rich synapse associated protein 1, cortactin SH3 domain-binding protein, Cortactin-binding protein 1, Proline-rich synapse-associated protein 1, cortact

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

N23B/49, monoclonal

species reactivity

mouse, human, rat

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_001004133

UniProt accession no.

Q9QX74

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... SHANK2(22941)

General description

Shank1, Shank2, and Shank3 make up a family of proteins that may function as molecular scaffolds in the postsynaptic density (PSD). Shank contains multiple domains for protein-protein interaction including ankyrin repeats, an SH3 domain, a PSD-95/Dlg/ZO-1 domain, a sterile α motif domain, and a proline-rich region. Alternative splicing in the Shank family may be a mechanism that regulates the molecular structure of Shank and the spectrum of Shank-interacting proteins in the PSDs of adult and developing brain.

Immunogen

Recombinant protein consisting of SH3/PDZ domain of rat Shank2.

Application

Detect pan-Shank using this Anti-pan-Shank Antibody, clone N23B/49 validated for use in WB, IH(P).
Immunohistochemistry Analysis: 1:500 dilution from a previous lot detected Shank in rat cerebellum tissue.

Western Blot Analysis: A previous lot of this antibody detected Shank in extracts of COS-1 cells transiently transfected with Shank1, Shank2 or Shank3 plasmids. Courtesy of James Trimmer, UC Davis/NIH NeuroMab Facility.
Research Category
Neuroscience
Research Sub Category
Synapse & Synaptic Biology

Quality

Evaluated by Western Blot in rat brain membrane tissue lysate.

Western Blot Analysis: 2 µg/mL of this antibody detected Shank on 10 µg of rat brain membrane tissue lysate.

Target description

~160 kDa observed. Other isoforms may be observed in some lysates.

Physical form

Format: Purified
Protein G
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Rat brain membrane tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Kenneth R Myers et al.
Frontiers in molecular neuroscience, 15, 1020949-1020949 (2022-10-18)
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Frontiers in synaptic neuroscience, 14, 995474-995474 (2022-10-18)
The Spontaneously Hypertensive Rat (SHR) has increased sympathetic drive to the periphery that precedes and contributes to the development of high blood pressure, making it a useful model for the study of neurogenic hypertension. Comparisons to the normotensive Wistar Kyoto
Yusuke Hatanaka et al.
Scientific reports, 5, 16102-16102 (2015-11-05)
Late-onset neurodegenerative diseases are characterized by neurological symptoms and progressive neuronal death. Accumulating evidence suggests that neuronal dysfunction, rather than neuronal death, causes the symptoms of neurodegenerative diseases. However, the mechanisms underlying the dysfunction that occurs prior to cell death

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