GC Analysis of Pesticides in Avocado on SLB®-5ms (20 m x 0.18 mm I.D., 0.36 μm) after QuEChERS Cleanup using Supel™ QuE Z-Sep+, Fast GC Analysis
Materials
SPE tube or plate
CONDITIONS
sample preparation
Dispersive (QuEChERS)
sample/matrix
Avocado spiked with pesticides at 20 ng/g
extraction tube
Supel QuE Acetate extraction tube (55234-U)
extraction process
add 25 mL of acetonitrile; shake for 1 minute; add contents of Supel QuE acetate extraction tube; shake immediately for 1 minute; centrifuge at 3200 rpm for 5 minutes
clean-up tube
Supel Que Z-Sep+ cleanup tube (55296-U)
clean-up process
transfer 3 mL of the acetonitrile layer into a Supel Que Z-Sep+ cleanup tube; shake for 1 minute; centrifuge at 3400 rpm for 3 minutes; draw off supernatant for LVI-GCMS analysis
column
SLB-5ms, 20 m x 0.18 mm I.D., 0.36 μm (28576-U)
oven
70 °C (2 min), 15 °C/min to 325 °C (6 min)
inj. temp.
60 °C (0.28 min), 600 °C/min to 325 °C (5 min)
detector
MSD
MSD interface
330 °C
carrier gas
helium, 1 mL/min, constant
injection
PTV solvent vent; 10 μL, injection dispense speed 15000 μL/min
liner
4 mm I.D. FocusLiner with taper
sample
pesticides spiked at 20 ng/g
Description
Analysis Note
About this analysis: This application demonstrates the use of dispersive SPE for the extraction of pesticides from Avocado. Cleanup is achieved using Z-Sep + for fat removal. FInal analysis of the extracts was done by LVI-GC/MS SIM on an SLB-5ms column.
Legal Information
SLB is a registered trademark of Merck KGaA, Darmstadt, Germany
Supel is a trademark of Sigma-Aldrich Co. LLC