Combined IL-15 and IL-12 drives the generation of CD34

Combined IL-15 and IL-12 drives the generation of CD34

Oncoimmunology (2015-07-04)

Jeannette Cany, Anniek B van der Waart, Jan Spanholtz, Marleen Tordoir, Joop H Jansen, Robbert van der Voort, Nicolaas M Schaap, Harry Dolstra

PMID26140247

ABSTRACT

Adoptive transfer of allogeneic natural killer (NK) cells represents a promising treatment approach against cancer, including acute myeloid leukemia (AML). Previously, we reported a cytokine-based culture method for the generation of NK cell products with high cell number and purity. In this system, CD34

MATERIALS

Product Number

Brand

Product Description

B6542

Sigma-Aldrich

Brefeldin A, ≥99% (HPLC and TLC), BioXtra, for molecular biology

B7651

Sigma-Aldrich

Brefeldin A, from Penicillium brefeldianum, ≥99% (HPLC and TLC)

B5936

Sigma-Aldrich

Brefeldin A, from Penicillium brefeldianum, Ready Made Solution, 10 mg/mL in DMSO

SRP3077

Sigma-Aldrich

IL-15 human, Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

I8648

Sigma-Aldrich

Interleukin-15 human, >97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture