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A BODIPY-luminol chemiluminescent resonance energy-transfer (CRET) cassette for imaging of cellular superoxide.

A BODIPY-luminol chemiluminescent resonance energy-transfer (CRET) cassette for imaging of cellular superoxide.

Organic & biomolecular chemistry (2014-12-17)

S Bag, J-C Tseng, J Rochford

ABSTRACT

Spectroscopic and in cellulo studies are here reported on the very first BODIPY-luminol chemiluminescent resonance energy-transfer (CRET) cassette where the luminol CL agent is covalently linked to the BODIPY energy-transfer acceptor in a molecular dyad. The efficiency of intramolecular CRET investigated for the BODIPY-luminol dyad was found to be 64% resulting in a dual emissive response. Successful in cellulo biochemiluminescence via CRET was achieved in PMA activated splenocytes.

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

VenPure^® SF, powder

Sigma-Aldrich

Boron, ≥95% (boron), amorphous powder

Supelco

Tetrahydrofuran, analytical standard

Supelco

Tetrahydrofuran, Selectophore^™, ≥99.5%

Sigma-Aldrich

Sodium borohydride solution, 2.0 M in triethylene glycol dimethyl ether

Supelco

Tetrahydrofuran, HPLC grade, ≥99.9%, inhibitor-free

Sigma-Aldrich

Tetrahydrofuran, anhydrous, ≥99.9%, inhibitor-free

Sigma-Aldrich

Sodium borohydride solution, ~12 wt. % in 14 M NaOH

Sigma-Aldrich

Boron, crystalline, 1 cm, 99.7% trace metals basis

Sigma-Aldrich

Tetrahydrofuran, anhydrous, contains 250 ppm BHT as inhibitor, ≥99.9%

Sigma-Aldrich

Phorbol 12-myristate 13-acetate, synthetic, ≥98.0% (TLC)

Sigma-Aldrich

Triethylamine, for amino acid analysis, ≥99.5% (GC)

Sigma-Aldrich

Triethylamine, BioUltra, ≥99.5% (GC)

Sigma-Aldrich

Triethylamine, ≥99.5%

Sigma-Aldrich

2,3-Dichloro-5,6-dicyano-p-benzoquinone, 98%

Sigma-Aldrich

Triethylamine, puriss. p.a., ≥99.5% (GC)

Sigma-Aldrich

Triethylamine, ≥99%

Sigma-Aldrich

Phorbol 12-myristate 13-acetate, ≥99% (TLC), film or powder

Sigma-Aldrich

Triethylamine, for protein sequence analysis, ampule, ≥99.5% (GC)

Sigma-Aldrich

Tetrahydrofuran, ACS reagent, ≥99.0%, contains 250 ppm BHT as inhibitor

Sigma-Aldrich

Triethylamine, ≥99.5%

Sigma-Aldrich

Tetrahydrofuran, contains 250 ppm BHT as inhibitor, ACS reagent, ≥99.0%

Supelco

Triethylamine, analytical standard

Supelco

Tetrahydrofuran, Pharmaceutical Secondary Standard; Certified Reference Material

Boron, monofilament, 10m, diameter 0.1mm

Boron, monofilament, 50m, diameter 0.2mm

Boron, monofilament, 200m, diameter 0.1mm

Boron, monofilament, 10m, diameter 0.2mm

Boron, monofilament, 20m, diameter 0.1mm

Boron, monofilament, 100m, diameter 0.1mm