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  • Elimination of protein band "edge tailing" in Weber-Osborn-type slab gel electrophoresis by glycerol.

Elimination of protein band "edge tailing" in Weber-Osborn-type slab gel electrophoresis by glycerol.

Analytical biochemistry (1993-09-01)
K Kubo
ABSTRACT

In sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) according to the method of Weber and Osborn, protein bands are often distorted by tailing at both ends, and the phenomenon is often called "edge tailing." This was eliminated by adding glycerol at a concentration of 10-15% (v/v) to the sample-well gel supplemented to form the sample wells. This simple modification made the protein bands as sharp and straight as those in SDS-PAGE according to the Laemmli procedure. The linearity of the semilogarithmic plot of mobilities versus molecular mass was better than that obtained by Laemmli-type SDS-PAGE.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Brilliant Blue R, 250, for microscopy
Sigma-Aldrich
Brilliant Blue R Staining Solution, suitable for (for immunoelectrophoresis protein staining)
Sigma-Aldrich
Brilliant Blue R, pure
Sigma-Aldrich
Brilliant Blue R, Dye content ~50 %, Technical grade
Sigma-Aldrich
Brilliant Blue R Concentrate, suitable for SDS-PAGE, methanol solution