- Method for measuring single-molecule adhesion forces and attachment lifetimes of protein-membrane interactions.
Method for measuring single-molecule adhesion forces and attachment lifetimes of protein-membrane interactions.
Methods in molecular biology (Clifton, N.J.) (2013-07-23)
Serapion Pyrpassopoulos, Henry Shuman, E Michael Ostap
PMID23868602
ABSTRACT
Many proteins that reside in the cytoplasm bind directly to cell membranes and play roles in signaling, adhesion, metabolism, cell structure, and cell motility. Several of these membrane-binding proteins, especially cytoskeletal proteins, have mechanical functions that result in the transmission of forces to the plasma membrane and organelle-membranes. Despite the importance of these interactions, remarkably little is known about the mechanical properties of the bonds between membranes and proteins. In this chapter, we describe a single-molecule, optical-trapping method for the measurement of protein-membrane adhesion forces and force-dependent attachment-lifetimes.
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