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PCR Master Mix Calculator

Performing calculations for large scale PCR reactions can be cumbersome and tedious. Ensure your success of scaled up reactions by using the PCR Master Mix Calculator. This online tool will calculate the amounts of components needed to create your PCR Master Mix.

Quickly calculate the amount of components needed to create your PCR Master Mix

PCR Master Mix Calculator

Composition of PCR reaction PCR MasterMix Formulation for PCR reactions


Template DNA µl Template DNA µl
PCR Buffer µl PCR Buffer µl
Forward Primer µl Forward Primer µl
Reverse Primer µl Reverse Primer µl
dNTP mix µl dNTP mix µl
DNA Polymerase µl DNA Polymerase µl
PCR grade Water µl PCR grade Water µl
Total Number of Reactions
Total PCR Reaction Volume µl
TOTAL VOLUME µl



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