SRP5161

4EBP1, GST tagged human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(s): BP-1, EIF4EBP1, MGC4316, PHAS-I
• CAS Number: 185703-54-2
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in E. coli
• Assay: ≥70% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• mol wt: ~40 kDa
• NCBI accession no.: NM_004095
• application(s): cell analysis
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... EIF4EBP1(1978)

Description

General description

Eukaryotic translation initiation factor 4E binding protein 1 (E1F4EBP1) is part of the translation repressor protein family. The gene encoding it is localized on human chromosome 8p11-p12.

Biochem/physiol Actions

Eukaryotic translation initiation factor 4E binding protein 1 (E1F4EBP1) is involved in protein synthesis, cell growth and survival. In the unphosphorylated form, it suppresses the translation initiation of capped mRNAs, whereas in phosphorylated form, it increases translation initiation. Phosphorylation of E1F4EBP1 releases it from eukaryotic translation initiation factor 4E (eIF4E) and this results in cap-dependent translation. The protein is also involved in tumorigenesis and the gene is overexpressed in various carcinomas. It is also a substrate for mTORC1 (mammalian target of rapamycin complex 1). In non-phosphorylated conditions, E1F4EBP1 interacts with p21 (cyclin-dependent kinase inhibitor) and enhances destabilization of p21.

Physical form

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Preparation Note

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable