Direkt zum Inhalt
Merck

HPLC Analysis of Protein Standards on Zenix® SEC-300 at Low Flow Rate

HPLC Analysis of Protein Standards on Zenix® SEC-300 at Low Flow Rate application for HPLC

Materialien

Analytische Säule

Produkt-Nr.
Beschreibung
Preisangaben

Z777033

Gelfiltrations-Säule Zenix® SEC-300, 3 μm

L × I.D. 30 cm × 7.8 mm, 3 μm particle size, stainless steel

Komponente der mobilen Phase

Produkt-Nr.
Beschreibung
Preisangaben

452289

Phosphorsäure

≥85 wt. % in H2O, ≥99.999% trace metals basis

Standard

Produkt-Nr.
Beschreibung
Preisangaben

M1882

Myoglobin aus Pferdeherz

≥90% (SDS-PAGE), essentially salt-free, lyophilized powder

U0750

Uracil

≥99.0%

T1001

Thyroglobulin aus Rinderschilddrüse

powder, ≥90% (agarose gel electrophoresis)

I4131

IgG aus Rattenserum

reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder

05470

Albumin aus Rinderserum

lyophilized powder, crystallized, ≥98.0% (GE)

CONDITIONS

column

Zenix SEC-300, 30 cm x 7.8 mm I.D., 3 μm (Z777033)

mobile phase

0.15 M potassium phosphate monobasic, pH 7.0 with phosphoric acid

flow rate

0.1 mL/min

pressure

173 psi (12 bar)

column temp.

25 °C

detector

UV, 215 nm

injection

4 μL

sample

protein standards

Beschreibung

Hinweis zur Analyse

Due to the low diffusion coefficents of proteins, optimal flow in size exclusion chromatography is often very low. However, the disadvantage is long run times. This example demonstrates both features.

Rechtliche Hinweise

Zenix is a registered trademark of Sepax Technologies

Chromatogram Search

Related Product Categories

HPLC ColumnsGC Columns & AccessoriesTLC Plates & AdsorbentsSolid Phase Microextraction (SPME)Solid Phase Extraction & QuEChERS

Related Applications

Small Molecule HPLCLarge Molecule HPLCGas Chromatography (GC)TLCSolid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS