- Kinetics of enzymatic degradation of high molecular weight polysaccharides through a nanopore: experiments and data-modeling.
Kinetics of enzymatic degradation of high molecular weight polysaccharides through a nanopore: experiments and data-modeling.
Analytical chemistry (2013-09-03)
Aziz Fennouri, Régis Daniel, Manuela Pastoriza-Gallego, Loïc Auvray, Juan Pelta, Laurent Bacri
PMID23992452
ZUSAMMENFASSUNG
The enzymatic degradation of long polysaccharide chains is monitored by nanopore detection. It follows a Michaelis-Menten mechanism. We measure the corresponding kinetic constants at the single molecule level. The simulation results of the degradation process allowed one to account for the oligosaccharide size distribution detected by a nanopore.
MATERIALIEN
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Produktbeschreibung
Sigma-Aldrich
Hyaluronidase aus Rindertestes, Type I-S, lyophilized powder, 400-1000 units/mg solid
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Hyaluronidase aus Rindertestes, Type IV-S, lyophilized powder (essentially salt-free), 750-3000 units/mg solid
Sigma-Aldrich
Hyaluronidase aus Rindertestes, Type IV-S, powder, suitable for mouse embryo cell culture, 750-3000 units/mg solid
Sigma-Aldrich
Hyaluronidase aus Rindertestes, Type VI-S, lyophilized powder, 3,000-15,000 units/mg solid