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  • Biomineralization in diatoms-phosphorylated saccharides are part of Stephanopyxis turris biosilica.

Biomineralization in diatoms-phosphorylated saccharides are part of Stephanopyxis turris biosilica.

Carbohydrate research (2012-12-12)
René Hedrich, Susanne Machill, Eike Brunner
ZUSAMMENFASSUNG

Diatoms-unicellular algae with silicified cell walls-have become model organisms for investigations of biomineralization processes. Numerous studies suggest the importance of biosilica-associated or even embedded biomolecules for the biosilica formation. Such molecules are peptides, polyamines, and even saccharides. However, the role of the latter class of biomolecules is only poorly understood yet. Therefore, we investigated the saccharide composition of the biosilica-associated organic material of the diatom Stephanopyxis turris. This species exhibits a considerably high saccharide content in its siliceous cell walls. Gas chromatography-mass spectrometry analysis revealed that mannose-6-phosphate is strongly associated to the cell walls. This phosphorylated saccharide has not yet been found in diatom biosilica. In vitro studies on the polyallylamine-induced silica precipitation were carried out in the presence of mannose-6-phosphate. Compared to inorganic phosphate, mannose-6-phosphate significantly influenced the precipitation behavior of this model system suggesting a possible contribution of mannose-6-phosphate to the biomineralization process of Stephanopyxis turris.

MATERIALIEN
Produktnummer
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Produktbeschreibung

Sigma-Aldrich
Poly(allylaminhydrochlorid), average Mw 50,000
Sigma-Aldrich
Poly(allylamin) -Lösung, 20 wt. % in H2O
Sigma-Aldrich
Poly(allylaminhydrochlorid), average Mw ~17,500 (GPC vs. PEG std.)
Sigma-Aldrich
D-Mannose-6-Phosphat Natriumsalz, ≥98% (HPLC)
Sigma-Aldrich
D-Mannose-6-Phosphat Dinatriumsalz Hydrat, ≥97.0% dry basis (enzymatic)
Sigma-Aldrich
Poly(allylamin) -Lösung, average Mw ~65,000, 10 wt. % in H2O