Direkt zum Inhalt
Merck
  • CHR721, interacting with OsRPA1a, is essential for both male and female reproductive development in rice.

CHR721, interacting with OsRPA1a, is essential for both male and female reproductive development in rice.

Plant molecular biology (2020-04-09)
Yushun Zhang, Qiong Chen, Guanlin Zhu, Fang Zhang, Xiaohua Fang, Haibo Ren, Jun'e Jiang, Fang Yang, Dechun Zhang, Fan Chen
ZUSAMMENFASSUNG

CHR721 functions as a chromatin remodeler and interacts with a known single-stranded binding protein, OsRPA1a, to regulate both male and female reproductive development in rice. Reproductive development and fertility are important for seed production in rice. Here, we identified a sterile rice mutant, chr721, that exhibited defects in both male and female reproductive development. Approximately 5% of the observed defects in chr721, such as asynchronous dyad division, occurred during anaphase II of meiosis. During the mitotic stage, approximately 80% of uninucleate microspores failed to develop into tricellular pollen, leading to abnormal development. In addition, defects in megaspore development were detected after functional megaspore formation. CHR721, which encodes a nuclear protein belonging to the SNF2 subfamily SMARCAL1, was identified by map-based cloning. CHR721 was expressed in various tissues, especially in spikelets. CHR721 was found to interact with replication protein A (OsRPA1a), which is involved in DNA repair. The expressions of genes involved in DNA repair and cell-cycle checkpoints were consistently upregulated in chr721. Although numerous genes involved in male and female development have been identified, the mode of participation of chromatin-remodeling factors in reproductive development is still not well understood. Our results suggest that CHR721, a novel gene cloned from rice, plays a vital role in both male and female reproductive development.

MATERIALIEN
Produktnummer
Marke
Produktbeschreibung

Supelco
Stärken (GO/P)-Assay-Kit, sufficient for 20 assays