G006123

HPLC Analysis of Morphine and Glucuronide Metabolites on Discovery^® HS F5

application for HPLC

Eigenschaften

• Methode(n): HPLC: suitable
• Testparameter: column: Discovery HS F5, 5 cm x 4.6 mm I.D., 5 μm particles (567513-U); mobile phase: [A] 10 mM ammonium acetate, pH 6.8 (unadjusted); [B] acetonitrile; gradient: 0% B for 1 min, to 30% B in 4 min, held 3 min; flow rate: 1 mL/min; column temp.: 40 °C; detector: UV, 280 nm; injection: 10 μL; sample: 50 μg/mL in final mobile phase
• Eignung: application for HPLC
• Anwendung(en): clinical; pharmaceutical (small molecule)

Beschreibung

Rechtliche Hinweise

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyt

Morphine 3-ß-D-glucuronide
Morphine-6-β-D-glucuronide solution, 100 μg/mL in methanol: water (1:1), ampule of 1 mL, certified reference material, Cerilliant^®
Morphine solution, 100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant^®