G003766

HPLC Analysis of Diazepam and Metabolites in Serum on Discovery^® C18 after SPE using Discovery^® DSC-8

application for SPE, application for HPLC

Eigenschaften

• Methode(n): HPLC: suitable; solid phase extraction (SPE): suitable
• Testparameter: sample preparation: SPE (Solid Phase Extraction); sample/matrix: goat serum spiked with diazepam and metabolites at 0.5 μg/mL and diluted 1:1 in 25 mM ammonium formate, pH 7.1; SPE well plate: Discovery DSC-8 96-well SPE Plate, 100 mg/well (575627-U); condition: 1 mL methanol followed by 1 mL DI water.; sample addition: 1 mL spiked goat serum; washing: 1 mL 25% methanol in 25 mM ammonium formate, pH 2.75; elution: 1 mL 60% methanol in 25 mM ammonium formate, pH 2.75; elution: Evaporate eluate with nitrogen purge (30 °C; ~15 min.). Reconstitute in 200 μL mobile phase.; column: Discovery C18, 5 cm x 4.6 mm I.D., 5 μm particles (504947); mobile phase: [A] Methanol; [B]10 mM ammonium acetate, pH 4.5;(45:55, A:B); flow rate: 1.5 mL/min; column temp.: 35 °C; detector: UV, 240 nm; injection: 25 μL
• Eignung: application for HPLC; application for SPE
• Anwendung(en): clinical; forensics and toxicology

Beschreibung

Rechtliche Hinweise

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany