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U8501

Uridine-5′-diphosphoglucose pyrophosphorylase from baker′s yeast

Type X, lyophilized powder, ≥40 units/mg protein

Synonym(s):

UTP:α-D-glucose-1-phosphate uridylyltransferase

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Size/SKUAvailabilityPrice
25 units
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$179.00
100 units
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$521.00
500 units
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$1,700.00
1000 units
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$2,490.00

About This Item

CAS Number:
EC Number:
UNSPSC Code:
12352204
EC Number:
232-811-2
NACRES:
NA.54
MDL number:
Specific activity:
≥40 units/mg protein
Biological source:
bakers yeast

$179.00


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biological source

bakers yeast

Quality Segment

type

Type X

form

lyophilized powder

specific activity

≥40 units/mg protein

composition

Protein, 30-60% modified Warburg-Christian

foreign activity

UDP-glucose dehydrogenase and galactose-1-phosphate uridyltransferase ≤0.1%, inorganic pyrophosphatase ≤0.5%

storage temp.

−20°C

General description

Uridine-5′-diphosphoglucose pyrophosphorylase (UDP-glc-PPase) is ubiquitous in plants, yeast, bacteria, and mammals. This enzyme is an octamer of eight identical sub-units.

Application

Uridine-5′-diphosphoglucose pyrophosphorylase from baker′s yeast has been used:
  • in the synthesis of uridine-5′-diphosphoglucose (UDP-Glc)-13C9
  • to quantify Suc synthase (SUS) enzyme activity in rice
  • to study the role of hexokinase and glycogen synthase controls the flux in frog oocytes[1]

Biochem/physiol Actions

Uridine-5′-diphosphoglucose pyrophosphorylase (UDP-glc-PPase) participates in catalyzing the synthesis of UDP-glucose. This enzyme requires divalent cations such as Mg2+, Ca2+, Mn2+, Ni2+ for its activity.

Physical form

Lyophilized, sulfate-free powder containing citrate buffer salt

Other Notes

One unit will form 1.0 μmole of glucose 1-phosphate from uridine-5′-diphosphoglucose and inorganic pyrophosphate per min at pH 7.6 at 25 °C.

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This Item
P7634P5381G4256
specific activity

≥40 units/mg protein

specific activity

≥500 units/mg protein

specific activity

≥400 units/mg protein (biuret)

specific activity

20-60 units/mg protein (modified Warburg-Christian)

biological source

bakers yeast

biological source

bakers yeast

biological source

bakers yeast

biological source

-

form

lyophilized powder

form

ammonium sulfate suspension

form

ammonium sulfate suspension

form

lyophilized powder

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

300

foreign activity

UDP-glucose dehydrogenase and galactose-1-phosphate uridyltransferase ≤0.1%, inorganic pyrophosphatase ≤0.5%

foreign activity

Glyceraldehyde-3-phosphate dehydrogenase ≤0.1%

foreign activity

-

foreign activity

6-phosphogluconate dehydrogenase ≤0.5%, UDP glucose pyrophosphorylase and galactokinase ≤0.2%


Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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