R8000

D-Ribulose 1,5-Diphosphate Carboxylase from spinach

partially purified powder, 0.01-0.1 unit/mg solid

• Synonym(e): 3-Phospho-D-glycerate carboxy-lyase(dimerizing), Rubisco
• CAS-Nummer: 9027-23-0
• EG-Nummer: 4.1.1.39 (BRENDA, IUBMB)
• UNSPSC Code: 12352204
• NACRES: NA.54
• MDL number: MFCD00130908
• Specific activity: 0.01-0.1 unit/mg solid
• Biological source: spinach

Eigenschaften

• biological source: spinach
• Quality Segment: 200
• form: partially purified powder
• specific activity: 0.01-0.1 unit/mg solid
• mol wt: 557 kDa
• storage temp.: −20°C

Beschreibung

General description

D-Ribulose 1,5-Diphosphate Carboxylase (RUBISCO) amounts to 50% of the total spinach leaves associated soluble protein.

Exists as a 557 kDa hexadecamer composed of eight heavy chains each with a molecular weight of approximately 56 kDa and eight light chains of molecular weight 14 kDa. Each molecule contains one magnesium ion.
pH optimum: ~7.9.
K_M for CO₂: ~0.45 mM.
Ribulose diphosphate becomes inhibitory at concentrations exceeding 0.7 mM. Orthophosphate and ammonium sulfate are competitive inhibitors. 3-Phosphoglycerate is a noncompetitive inhibitor.

Application

D-Ribulose 1,5-Diphosphate Carboxylase from spinach has been used:

• as a test protein in pepsin digestion studies
• as an innocuous or non-hazardous protein sample to test its effect on human intestinal epithelial cell lines
• in isothermal titration calorimetry (ITC), and radiolabeled binding assays with abscisic acid

Biochem/physiol Actions

D-Ribulose 1,5-Diphosphate Carboxylase (RUBISCO) depends on Rubisco activase and chaperones for activation. It participates in plant photorespiration events by catalyzing the carboxylation and oxygenation of ribulose-1,5-bisphosphate. Abscisic acid inhibits the carboxylation activity of Rubisco.

Other Notes

One unit will convert 1.0 μmole of D-RuDP and CO₂ to 2.0 μmoles of D-3-phosphoglycerate per min at pH 7.8 at 25°C.

Sicherheitshinweise

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)