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PCRISPR002

Sigma-Aldrich

Mouse CRISPR Activation Caprano Library

Synonym(e):

CRISPR activation library, Mouse CRISPR library

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About This Item

UNSPSC-Code:
41105904
NACRES:
NE.02

Verpackung

pkg of 10 vials (5x200µL aliquots of each component)

Konzentration

≥5x108 VP/ml (via p24 Assay)

Anwendung(en)

CRISPR

Versandbedingung

dry ice

Lagertemp.

−70°C

Allgemeine Beschreibung

The mouse CRISPR activation library (Caprano) activates over 22,000 mouse genes and is used for genome-wide activation screening. The library is designed to be compact and efficient to maximize screening efficiency and performance, as published by Sanson, K.R., et al. Nat Commun 9, 5416 (2018).

Custom pools for follow-up screening or 10x Genomics Compatible CRISPR pools are also available by contacting your local sales representative.

Anwendung

  • Functional Genomics/Target Validation
  • Focused forward genetic activation screening
  • Validated positive and negative controls

Leistungsmerkmale und Vorteile

  • Focus on your research, and we will generate your lentivirus screening library
  • Compatible with SAMHELPERV for consistent effector expression across a wide variety of cell lines dCas9-VP64-Blasticidin MS2-P65-HSF1-Hygromycin
  • Targeting Genes22,774 (Set A), 22,658 (Set B)
  • Compact Libary with gRNAs 67,187 (Set A), 66,889 (Set B)
  • Built-in Controls 500, unique non-targeting controls are in each of the two half-libraries (Set A and Set B)

Komponenten

2 Subpools with a minimum concentration of 5x108 VP/mL(via p24 assay)
PCRISPR002A - Mouse CRISPR Activation Caprano Library Set A
PCRISPR002B - Mouse CRISPR Activation Caprano Library Set B

Prinzip

The most effective CRISPRa system uses the CRISPR Synergistic Activation Mediator (SAM) complex. This platform combines VP64-dCas9 with a modified guide. In addition, the gRNA contains two aptamers that recruit additional co-activators, p65 and HSF1, enhancing target gene activation.

CRISPRa can also serve as a complementary approach to loss-of-function (LOF) genetic screens by enriching for a different set of genes responsible for a phenotype CRISPRa screens show little-to-no off-target activity and can be used to study genes that are difficult to characterize using only LOF approaches because of their high copy number.

Rechtliche Hinweise

CRISPR USE LICENSE AGREEMENT

LENTIVIRAL, WPRE AND EVROGEN FLUOROPHORE LICENSES

Piktogramme

Exclamation mark
GHS07

Signalwort

Warning

H-Sätze

H317

Gefahreneinstufungen

Skin Sens. 1

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

Kendall R Sanson et al.
Nature communications, 9(1), 5416-5416 (2018-12-24)
The creation of genome-wide libraries for CRISPR knockout (CRISPRko), interference (CRISPRi), and activation (CRISPRa) has enabled the systematic interrogation of gene function. Here, we show that our recently-described CRISPRko library (Brunello) is more effective than previously published libraries at distinguishing

Artikel

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