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N7653

p-Nitrophenyl Phosphate Liquid Substrate System

alkaline phosphatase substrate, liquid

Synonym(e):

4-Nitrophenyl phosphate disodium salt solution, pNPP

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100 ML

€ 262,00

€ 262,00


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Über diesen Artikel

Lineare Formel:
O2NC6H4OP(O)(ONa)2
CAS-Nummer:
Molekulargewicht:
263.05
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.83
MDL number:

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Produktname

p-Nitrophenyl Phosphate Liquid Substrate System, liquid

form

liquid

Quality Level

shipped in

wet ice

storage temp.

−20°C

SMILES string

[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

InChI

1S/C6H6NO6P.2Na/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;/h1-4H,(H2,10,11,12);;/q;2*+1/p-2

InChI key

VIYFPAMJCJLZKD-UHFFFAOYSA-L

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Dieser Artikel
P7998B3804487655
Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

100

form

liquid

form

liquid

form

liquid

form

solid

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

wet ice

shipped in

-

shipped in

-

shipped in

ambient

General description

The p-Nitrophenyl Phosphate (pNPP) Liquid Substrate System combines p-nitrophenyl phosphate, buffer, and the required magnesium cations in a convenient, ready-to-use, single solution reagent. Producing a soluble end product, pNPP is the substrate of choice in alkaline phosphatase enzyme immunoassays.[1]

Application

p-Nitrophenyl Phosphate Liquid Substrate System has been used as a substrate to determine the activity of alkaline phosphatase in alkaline phosphatase assay.
Substrate for alkaline phosphatase that yields a soluble yellow end product that may be read at 405 nm. The reaction may be stopped with 3N NaOH and read at 405 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications.

Biochem/physiol Actions

4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) is a non-proteinaceous and non-specific substrate for determining protein tyrosine phosphatase activity. Hydrolysis of pNPP produces p-nitrophenol. It is a common chromogenic substrate for alkaline phosphatase.

Physical form

Ready-to-use.

Disclaimer

Stable at least one year when stored below 0 °C.

pictograms

Health hazardCorrosion

signalword

Danger

Hazard Classifications

Eye Dam. 1 - Met. Corr. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 2 Oral

target_organs

Kidney,Liver,Blood

Lagerklasse

12 - Non Combustible Liquids


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We have recently described a substrate amplification system, based on the method of Self, which increases the sensitivity of alkaline phosphatase (AP)-dependent enzyme-linked immunosorbent assays (ELISA) by a factor of 30-50. This increase is achieved by having AP, the primary

Artikel

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Verwandter Inhalt

Global Trade Item Number

SKUGTIN
N7653-100ML04061838256126

Questions

1–4 of 4 Questions  
  1. What type of application can Product N7653, p-Nitrophenyl Phosphate Liquid Substrate System, be used in?

    1 answer
    1. This product is recommended for ELISA (microwell) procedures and is not recommended for membrane applications.

      Helpful?

  2. What is the concentration of Product N7653, p-Nitrophenyl Phosphate Liquid Substrate System?

    1 answer
    1. The concentration of this product is considered proprietary.

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  3. How should Product N7653, p-Nitrophenyl Phosphate Liquid Substrate System, be used?

    1 answer
    1. Information on usage is found in our product information sheet (under Documents, above).

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  4. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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