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Merck

M4892

SAFC

McCoy 5A-Medium

Modified, with L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Arzneimittelherstellung

Synonym(e):

5A basal medium

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About This Item

MDL-Nummer:
UNSPSC-Code:
12352207
NACRES:
NA.71

Qualitätsniveau

Form

powder

Methode(n)

cell culture | mammalian: suitable

Komponenten

phenol red: 0.011 g/L
L-glutamine: 0.21915 g/L
glucose: 3.0 g/L (Dextro)
NaHCO3: no

Versandbedingung

ambient

Lagertemp.

2-8°C

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Allgemeine Beschreibung

McCoy′s 5A Medium is recommended to produce viruses in primary cell cultures.

Anwendung

McCoy′s 5A Medium has been used for culturing:
  • human colon carcinoma cell lines (HCT116, RKO, FET, CBS, HCT116b, and TENN) to study the function of leucine-rich repeat-containing G-protein-coupled receptor (LGR5) in colon cancer metastasis
  • human colorectal HCT116 cells to study miR-224 over-expression in cancer
  • osteoblasts to study ion reactivity of calcium-deficient hydroxyapatite in standard cell culture media

Biochem./physiol. Wirkung

McCoy′s 5A-Medium wurde ursprünglich zur Unterstützung von Lebertumorzellen durch die Modifikation der in BME vorkommenden Aminosäuren entwickelt. Diese Formulierung wird auch zur Unterstützung des Wachstums in Primärkulturen von Knochenmark, Haut, Gingiva, Niere, Omentum, Nebenniere, Lunge, Milz, Rattenembryos und anderen Zelltypen verwendet.

Menge

Formulated to contain 11.9 grams of powder per liter of medium.

Rekonstituierung

Supplement with 2.2 g/L sodium bicarbonate.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Yan Zhang et al.
Oncotarget, 8(53), 91134-91149 (2017-12-07)
Human osteosarcoma is considered a malignant tumor with poor prognosis that readily metastasizes. Tetrahydrocurcumin (THC) has been reported to have anti-tumor activity in numerous tumors. In addition, hypoxia-inducible factor-1α (HIF-1α) has been demonstrated to be associated with tumor metastasis by
Yu Wang et al.
PloS one, 8(7), e68744-e68744 (2013-08-08)
MicroRNA-224 (miR-224) is frequently over-expressed in liver and colorectal cancers. We and others have previously described the role of miR-224 over-expression in cell proliferation in vitro but we have yet to identify the relevant miR-224 direct target. In this study
Ying Liu et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 44(4), 1396-1410 (2017-12-05)
Osteosarcoma (OS) is the most common primary malignant tumor of bone tissue. Although treatment effectiveness has improved, the OS survival rate has fluctuated in recent years. Andrographolide (AG) has been reported to have antitumor activity against a variety of tumors.
Archana Kumari et al.
The EMBO journal, 39(14), e104006-e104006 (2020-06-23)
Cellular studies of filamentous actin (F-actin) processes commonly utilize fluorescent versions of toxins, peptides, and proteins that bind actin. While the choice of these markers has been largely based on availability and ease, there is a severe dearth of structural
Xiang-Ping Liu et al.
Journal of experimental & clinical cancer research : CR, 28, 52-52 (2009-04-21)
RhoA and RhoC are deregulated by over expression in many human tumors, including colorectal cancer. Some reports show that they play a pivotal role in the carcinogenesis, tumor development and infiltration metastasis. In this study, for the first time we

Artikel

McCoy's 5A Modified Media Formulation

Protokolle

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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