Direkt zum Inhalt
Merck
Alle Fotos(1)

Dokumente

G2751

Sigma-Aldrich

Glutamat-Oxalessig-Transaminase Zubereitung aus Schweineherz

Type I, ammonium sulfate suspension, 200-500 units/mg protein

Synonym(e):

L-Aspartate:2-oxoglutarate aminotransferase, Aspartat-Aminotransferase, GOT

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(1)

About This Item

CAS-Nummer:
9000-97-9
EC-Nummer:
2.6.1.1 (BRENDA, IUBMB)
EG-Nummer:
232-571-9
MDL-Nummer:
MFCD00131191
UNSPSC-Code:
12352204
NACRES:
NA.54

Typ

Type I

Form

ammonium sulfate suspension

Spezifische Aktivität

200-500 units/mg protein

Fremdaktivität

glutamic-pyruvic transaminase ≤0.03%
lactic dehydrogenase ≤0.01%
malic dehydrogenase ≤0.01%

Versandbedingung

wet ice

Lagertemp.

2-8°C

Allgemeine Beschreibung

Research area: Cellsignaling. Glutamic-Oxalacetic Transaminase is found in all tissues and is predominant in the liver and skeletal muscle. It is a prototype of fold-type I pyridoxal 5′-phosphate (PLP)-enzymes. Glutamic-oxalacetic transaminase is a homodimer containing large and small domains in each subunit.

Anwendung

Glutamic-OxalaceticTransaminase from porcine heart is suitable for the preparation ofphenylalanine amino acid. The immobilized form of this enzyme maybe used incomparison studies between soluble and immobilized forms in terms of reactionefficiency and stability.
Glutamic-Oxalacetic Transaminase from porcine heart has been used:
  • in an enzyme-coupled assay for evaluating L-asparaginase enzyme activity
  • in microtiter plate format enzyme-based assay to estimate malic acid content in berry samples
  • as a supplement in homogenization buffers for the isolation of heart mitochondrial membranes in the presence of oxaloacetate (OAA)-depleting system

Biochem./physiol. Wirkung

Glutamic-oxalacetic transaminase or aspartate aminotransferase (AAT) catalyzes the interconversion of L-aspartate and α-ketoglutarate with oxalacetate and L-glutamate. This reversible transaminase reaction is dependent on pyridoxal 5′-phosphate (PLP). Glutamic-oxalacetic transaminase maintains the nitrogen currency for metabolism by generating L-glutamate. Elevated serum levels of glutamic-oxalacetic transaminase are indicated in myocardial infarction, liver diseases, and some renal diseases.

Einheitendefinition

Eine Unit verwandelt 1 μmol α-Ketoglutarat zu L-Glutamat pro Minute bei einem pH von 7,5 und 37 °C, in Gegenwart von L-Asparaginsäure. Eine Unit entspricht ~2,000 O.D. (Karmen) Units bei 25 °C.

Physikalische Form

Suspension in 3.0 M (NH4)2SO4, enthält 0.05 M Maleat und 2.5 mM α-Ketoglutarat, pH 6.0

Hinweis zur Analyse

Proteingehalt mittels Biuret-Reaktion bestimmt.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Anna Stepanova et al.
Biochimica et biophysica acta, 1857(9), 1561-1568 (2016-06-12)
Mitochondrial Complex II is a key mitochondrial enzyme connecting the tricarboxylic acid (TCA) cycle and the electron transport chain. Studies of complex II are clinically important since new roles for this enzyme have recently emerged in cell signalling, cancer biology
Clinical biochemistry and hematology
The laboratory rabbit, guinea pig, hamster, and other rodents., 57-116 (2012)
The Study of Serum GOT (Glutamic Oxalacetic Transaminase) Activity and Some kinetic Parameters in Patient with Pulmonary Tuberculosis
Ismahil PA and Hassan LM
Biological Chemistry, 23, 159-169 (2017)
Pyridoxal 5-phosphate-dependent enzymes: catalysis, conformation, and genomics
Comprehensive Natural Products II (2010)
Michael D Toney
Archives of biochemistry and biophysics, 544, 119-127 (2013-10-15)
Aspartate aminotransferase (AAT) is a prototypical pyridoxal 5'-phosphate (PLP) dependent enzyme that catalyzes the reversible interconversion of l-aspartate and α-ketoglutarate with oxalacetate and l-glutamate via a ping-pong catalytic cycle in which the pyridoxamine 5'-phosphate enzyme form is an intermediate. There
Alle zugehörigen Dokumente anzeigen

Artikel

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

Setzen Sie sich mit dem technischen Dienst in Verbindung.