This product is an Immunoprecipitation kit that includes both glycine and albumin in the protocol. The albumin is utilized as a blocking agent, and the glycine is used to stop the cross-linking reaction. Please see the link below to review the complete product information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/308/933/chp2bul.pdf
CHP2NC
Imprint® Ultra Chromatin Immunoprecipitation Kit, Without Controls
ChIP kit for maximum sensitivity, compatible with Next-Gen sequencing
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NACRES:
NA.54
UNSPSC Code:
41116158
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Dieser Artikel | CHP1 | CHROP | RIP |
|---|---|---|---|
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
General description
The Imprint Ultra Chromatin IP Kit is Sigma′s second generation chromatin immunoprecipitation (ChIP) kit developed for maximum sensitivity and optimum next-generation sequencing results. It provides a complete solution for Chromatin Immunoprecipitation, including columns and reagents for DNA purification. The kit allows researchers to explore the genome-wide binding sites of low abundance transcription factors (TFs), as well as novel histone modifications. It is optimized for ChIP reactions with chromatin from 106 cells (up to ~50 μg DNA), and can also be scaled up (or several preparations pooled) to accommodate 108 cells for genome-wide binding studies in ChIP-chip and ChIP-Seq applications.
Application
- Suitable for downstream applications
- Individual target characterization to genome-wide profiling techniques
- Characterization of signal transduction pathways
- Verification of ChIp-chIP and ChIP-seq data
Features and Benefits
- Use successfully CHiP′ed DNA associated with low abundance, medium, and highly expressed transcription factors, as well as histone modifications
- Greater capacity - can be used over a wide range of cell numbers ranging from 2-10 × 106
- Maximum sensitivity - capable of detecting low abundance transcription factors with as little as 2 × 106 cells
- Employs DNA-Blocked "Staph-Seq" for IP (immunoprecipitation), minimizing contaminating Staph A DNA in downstream ChIP-Seq applications.
Legal Information
Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany
Nur Kit-Komponenten
Produkt-Nr.
Beschreibung
- Sodium dodecyl sulfate solution, 10% in 18 megohm water
- Monoclonal Anti-POLR2A antibody produced in mouse, clone 1F17, purified immunoglobulin, buffered aqueous solution
Kit-Komponenten auch einzeln erhältlich
Produkt-Nr.
Beschreibung
SDB
- S6576ChIP Next Gen Seq Sepharose™
- I8896IGEPAL® CA-630, Molecular Biology
- S5150Sodium chloride solution, 5 M in H2O, BioReagent, Molecular Biology
- W4502Water, Nuclease-Free Water, for Molecular Biology
- I5006IgG from rabbit serum, reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder
- I5381IgG from mouse serum, reagent grade, ≥95% (SDS-PAGE), lyophilized powder
- A7638Bovine Serum Albumin, lyophilized powder, essentially globulin free, ≥99% (agarose gel electrophoresis)
- A84564-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride, ≥97.0% (HPLC)
- D9156Deoxyribonucleic acid, single stranded from salmon testes, For hybridization
- M7023Anti-Mouse IgG (whole molecule) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
- P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution
- R4642Ribonuclease A from bovine pancreas, (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)
Alle anzeigen (12)
signalword
Danger
target_organs
Central nervous system
Lagerklasse
5.1A - Strongly oxidizing hazardous materials
wgk
WGK 3
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - ED ENV 1 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Ox. Liq. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3
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Comparison of sample preparation methods for ChIP-chip assays.
O'Geen, H., et al.
Biotechniques, 41, 557-557 (2006)
Chromatin isolation by RNA purification (ChIRP).
Chu, C., et al.
Journal of Visualized Experiments (2012)
Genomic maps of long noncoding RNA occupancy reveal principles of RNA-chromatin interactions.
Chu, C., et al.
Molecular Cell (2011)
Genetic Framework for GATA Factor Function in Vascular Biology
Linnemann A.K., et al.
Proceedings of the National Academy of Sciences of the USA, 1, 13641-13646 (2011)
Yoon-A Kang et al.
Molecular and cellular biology, 32(1), 226-239 (2011-10-26)
Developmental and homeostatic remodeling of cellular organelles is mediated by a complex process termed autophagy. The cohort of proteins that constitute the autophagy machinery functions in a multistep biochemical pathway. Though components of the autophagy machinery are broadly expressed, autophagy
Protokolle
Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.
Verwandter Inhalt
Protein and nucleic acid interaction reagents and resources for investing protein-RNA, protein-DNA, and protein-protein interactions and associated applications.
Interaktionsreagenzien für Protein und Nukleinsäure und Ressourcen für die Untersuchung von Protein-RNA-, Protein-DNA- und Protein-Protein-Interaktionen und damit verbundene Anwendungen.
Imprint® Ultra Chromatin IP Kit: for Successful ChIP-Seq with a Low Abundance Transcription Factor
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