Direkt zum Inhalt
Merck

Fortfahren mit

E7889

Ethylendiamintetraessigsäure Dinatriumsalz -Lösung

Molecular Biology, 0.5 M in H2O, DNase, RNase, NICKase and protease, none detected

Synonym(e):

EDTA Dinatriumsalz, EDTA-Na2, Ethylendinitrilo-tetraessigsäure Dinatriumsalz

Anmelden zur Ansicht der Organisations- und Vertragspreise.

Größe auswählen

Ansicht ändern
PackungsgrößeSKUVerfügbarkeitPreis

Über diesen Artikel

Lineare Formel:
[-CH2N(CH2CO2Na)CH2CO2H]2
CAS-Nummer:
Molekulargewicht:
336.21
UNSPSC Code:
12352107
NACRES:
NA.31
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
3822669
Preise und Verfügbarkeit sind derzeit nicht verfügbar.
Technischer Dienst
Benötigen Sie Hilfe? Unser Team von erfahrenen Wissenschaftlern ist für Sie da.
Unterstützung erhalten


grade

Molecular Biology

Quality Segment

sterility

0.2 μm filtered

form

liquid

reaction suitability

reagent type: chelator

concentration

0.5 M in H2O

pH

7.5-8.5

suitability

suitable for molecular biology

application(s)

microbiology

foreign activity

DNase, RNase, NICKase and protease, none detected

SMILES string

[Na+].[Na+].OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O

InChI

1S/C10H16N2O8.2Na/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;/q;2*+1/p-2

InChI key

ZGTMUACCHSMWAC-UHFFFAOYSA-L

General description

Ethylendiamintetraessigsäure (EDTA) ist eine mehrwertige Säure. Die vier Carbonsäure- und zwei Aminogruppen mit Valenzelektronen von EDTA können Calcium und verschiedene andere Metallionen chelatisieren.
Ethylendiamintetraessigsäure-Dinatriumsalzlösung ist ein für die Molekularbiologie geeigneter Chelatbildner.

Application

Ethylendiamintetraessigsäuredinatriumsalz-Lösung wird wie folgt verwendet:
  • in Tris-Salzsäure (HCl) für den Aufschluss von Proteinase-K zum Bestimmen der Menge von DNA in der Corneaprobe
  • mit Papain für den Aufschluss von Corneaproben zur Quantifizierung des Glykosaminoglykan(GAG)-Gehalts
  • bei der Vorbehandlung zum Ablösen von Epithelzellen, die auf eine Konfluenz von 80 % gezüchtet wurden
  • zum Herstellen von Stammlösungen für Lysepuffer
  • bei der Herstellung von Harnstofflysepuffer

Features and Benefits

Molekularbiologische Qualität

Preparation Note

Aufbereitet in 18-Megohm-Wasser mit EDTA der molekularbiologischen Güteklasse (E 5134)

Other Notes

Füllen Sie bitte dieses Formular aus, um weitere Informationen zu unserem Sortiment an Biochemikalien zu erhalten.

Ähnliche Artikel vergleichen

Vollständigen Vergleich anzeigen

Unterschiede anzeigen

1 of 1

Dieser Artikel
036902854ED4SS
grade

Molecular Biology

grade

Molecular Biology

grade

-

grade

-

Quality Level

300

Quality Level

200

Quality Level

200

Quality Level

300

application(s)

microbiology

application(s)

-

application(s)

-

application(s)

-

pH

7.5-8.5

pH

8.0±0.2

pH

-

pH

-

form

liquid

form

liquid

form

solution

form

crystalline

sterility

0.2 μm filtered

sterility

-

sterility

-

sterility

-


Still not finding the right product?


pictograms

Health hazard

signalword

Warning

hcodes

Hazard Classifications

STOT RE 2 Inhalation

target_organs

Respiratory Tract

Lagerklasse

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number

Die passende Version wird nicht angezeigt?

Wenn Sie eine bestimmte Version benötigen, können Sie anhand der Lot- oder Chargennummer nach einem spezifischen Zertifikat suchen.

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen


Protokolle

Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.

Artikel

ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.


Marta Filipiak et al.
Fish physiology and biochemistry, 38(2), 355-362 (2011-05-27)
The aim of this study was to compare DNA content in hepatocyte and erythrocyte nuclei of the European sunbleak, Leucaspius delineatus, in relation to nuclear and cell size by means of flow cytometry and fluorescence microscopy. The DNA standards, chicken
Yi-Yuan Ke et al.
Foods (Basel, Switzerland), 10(1) (2021-01-21)
Isothiocyanates (ITCs) are important functional components of cruciferous vegetables. The principal isothiocyanate molecule in broccoli is sulforaphane (SFN), followed by erucin (ERN). They are sensitive to changes in temperature, especially high temperature environments where they are prone to degradation. The
Yongchao Dou et al.
Cell, 180(4), 729-748 (2020-02-16)
We undertook a comprehensive proteogenomic characterization of 95 prospectively collected endometrial carcinomas, comprising 83 endometrioid and 12 serous tumors. This analysis revealed possible new consequences of perturbations to the p53 and Wnt/β-catenin pathways, identified a potential role for circRNAs in



Global Trade Item Number

SKUGTIN
E7889-100ML04061838255334
E7889-1L04061838110152

Questions

1–8 of 8 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Can a solution of EDTA disodium salt, Product E7889, be autoclaved?

    1 answer
    1. Yes, solutions of EDTA may be autoclaved.

      Helpful?

  4. How many divalent cations does Product E7889, EDTA disodium salt, complex with?

    1 answer
    1. EDTA forms 1:1 complex with divalent cations.

      Helpful?

  5. What is the pH of E7889, EDTA disodium salt solution?

    1 answer
    1. E7889 has a pH specification of 7.5 to 8.5 at 25 °C.

      Helpful?

  6. At what concentration should I use Product E7889, EDTA disodium salt, as an anticoagulant?

    1 answer
    1. The optimal concentration of EDTA to be used as an anticoagulant is 1.5 mg of EDTA disodium salt per ml of blood or 1-2 drops of a 2% EDTA solution per ml of blood.

      Helpful?

  7. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  8. What type of biological activity does Product E7889, EDTA disodium salt, exhibit?

    1 answer
    1. EDTA is a metal ion-dependent enzyme inhibitor. It may be used as a metalloprotease inhibitor at a concentration of 1-10 μm, or as a DNAse I inhibitor at a concentration of 1-10 mM.

      Helpful?

Reviews

No rating value

Active Filters