MABF157E

Anti-CD3e (Mouse), clone 145-2C11, biotin conjugate Antibody

clone 145-2C11, from hamster(Armenian), purified by affinity chromatography

• Synonym(e): T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3e
• UNSPSC Code: 12352203
• NACRES: NA.45
• eCl@ss: 32160702
• Conjugate: biotin conjugate
• Clone: 145-2C11, monoclonal
• Application: FACS
• Citations: -

Eigenschaften

• biological source: hamster (Armenian)
• Quality Segment: 100
• conjugate: biotin conjugate
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 145-2C11, monoclonal
• purified by: affinity chromatography
• species reactivity: mouse
• packaging: antibody small pack of 25 μg
• technique(s): flow cytometry: suitable
• isotype: IgG
• UniProt accession no.: P22646
• target post-translational modification: unmodified
• Gene Information: mouse ... Cd3E(12501)

Beschreibung

General description

The 145-2C11 antibody is specific for mouse CD3e, also known as CD3 epsilon, a 20 kDa subunit of the T cell receptor complex, along with CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR), as well as CD3 zeta chain, to form the T cell receptor – CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. Such interactions promote T cell receptor signaling (T cell activation) and can result in a number of cellular responses including proliferation, differentiation, production of cytokines or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells.The 145-2C11 antibody is a widely used phenotypic marker for mouse T cells. In addition, binding of 145-2C11 antibody to CD3e can induce cell activation. A recent publication of the crystal structure of a murine CD3e-mitogenic antibody complex provides further insight into the action of commonly used agonist antibodies (Fernandes, R.A. et al. 2012. J. Biol. Chem. 287: 13324-13335).

Application

Anti-CD3e (Mouse), clone 145-2C11, biotin conjugate Antibody detects level of CD3e and has been published and validated for use in Flow Cytometry.

Analysis Note

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.125 μg from a representative lot detected CD3e in one million C57Bl/6 splenocytes.

Other Notes

Concentration: Please refer to lot specific datasheet.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable