ABS1614

Anti-Ric-8B/Synembryn-B Antibody

serum, from rabbit

• Synonym(e): Synembryn-B, Protein Ric-8B, Ric-8B, Ric-8B/Synembryn-B
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IP, WB
• Citations: -

Eigenschaften

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: mouse, rabbit, human
• technique(s): immunoprecipitation (IP): suitable, western blot: suitable
• NCBI accession no.: NP_898995
• UniProt accession no.: Q80XE1
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... RIC8B(55188)

Beschreibung

General description

Synembryn-B (UniProt Q80XE1; also known as Protein Ric-8B, Resistance to inhibitors of cholinesterase 8B) is encoded by the Ric8b (also known as BC051080) gene (Gene ID 237422) in murine species. Ric-8A and Ric-8B stimulate nucleotide exchange of all Gα subunit classes by stabilizing the Gα nucleotide-free transition state. Ric-8A acts upon Gαi/q/13, while Ric-8B targets Gαs/olf. In addition to their function as guanine nucleotide exchange factors (GEFs), Ric proteins also act as molecular chaperones that directly participate in the posttranslational folding of nascent Gα subunits to promote G-protein biosynthesis and membrane association. Ric-8 knockdown reduces Gα steady-state abundances and overexpression of Ric-8 proteins enhances cellular Gα levels. In Ric-8A– or Ric-8B–delicient ES cells, newly produced Gα subunits are defective in the initial association with an internal membrane, resulting in a ∼10-fold faster turned over rate than in WT cells.

~63 kDa observed. Uncharacterized band(s) may appear in some lysates.

Immunogen

Recombinant protein corresponding to mouse Ric-8B/Synembryn-B.

Application

This Anti-Ric-8B/Synembryn-B Antibody is validated for use in Western Blotting and Immunoprecipitation for the detection of Ric-8B/Synembryn-B.

Western Blotting Analysis: A representative lot detected Ric-8B protein in murine embryonic stem (mES) cells (mESCs) derived from wild-type (WT) and Ric-8A−/− mice, but not mESCs from Ric-8B−/− mice (Gabay, M., et al. (2011). Sci Signal.4(200):ra79).
Western Blotting Analysis: A representative lot detected the endogenous Ric-8B protein in rabbit reticulocyte lysates (RRL) as well as recombinant murine Ric-8B protein (Chan, P., et al. (2013). Proc Natl Acad Sci U S A. 110(10):3794-3799).
Immunoprecipitation Analysis: A representative lot immunoprecipitated the endogenous Ric-8B protein from rabbit reticulocyte lysates/RRL (Chan, P., et al. (2013). Proc Natl Acad Sci U S A. 110(10):3794-3799).

Biochem/physiol Actions

Expected to react with all murine and human spliced isoforms (UniProt Q80XE1 & Q9NVN3).

Analysis Note

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 1:1,000 dilution of this antibody detected Ric-8B/Synembryn-B in 10 µg of Jurkat cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable