ABE602

Anti-SATB1 Antibody

from rabbit, purified by affinity chromatography

• Synonym(e): DNA-binding protein SATB1, Special AT-rich sequence-binding protein 1
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: human
• Methode(n): immunofluorescence: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• UniProt-Hinterlegungsnummer: Q01826
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... SATB1(6304)

Beschreibung

Allgemeine Beschreibung

Special AT-rich sequence-binding protein 1 (SATB1) contains 2 CUT DNA-binding domains and one homeobox DNA binding domain belonging to the CUT homeobox family. SATB1 contributes to the initiation of X inactivation mediated by Xist RNA that occurs during embryogenesis and in lymphoma. SATB1 is organized into a cage-like network anchoring loops of heterochromatin and regulates gene expression through chromatin remodeling/HDAC (histone deacetylase complex), and transcription factors recruitment. SATB1 is predominantly expressed in thymus. Recent studies show that SATB1 is necessary for breast cancer cells to become metastatic.

Spezifität

This antibody recognizes the C-terminus of SATB1.

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human SATB1.

Anwendung

Research Category
Epigenetik & nukleäre Funktionen

Research Sub Category
Transkriptionsfaktoren

Detect Special AT-rich sequence-binding protein 1 using this rabbit polyclonal antibody, Anti-SATB1 Antibody validated for use in western blotting, IHC (Paraffin) & Immunofluorescence.

Immunohistochemistry Analysis: 5 µg/mL from a representative lot detected SATB1 in human brain tissue.

Immunofluorescence Analysis: 20 μg/mL from a representative lot detected SATB1 in human brain cells.

Qualität

Evaluated by Western Blotting in A20 cell lysate.

Western Blotting Analysis: 4 µg/mL of this antibody detected SATB1 in 15 µg of A20 cell lysate.

Zielbeschreibung

~90 and ~98 kDa observed. Uniprot describes two isoforms at ~86 kDa and ~89 kDa Uncharacterized bands may appear in some lysate(s).

Physikalische Form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Hinweis zur Analyse

Control
A20 cell lysate

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable