5.09228

Y-27632

≥95% (HPLC), liquid, Rho kinase inhibitor, Calbiochem

• Synonym(e): InSolution Y-27632, Sterile-Filtered, Cell Culture-Tested
• Empirische Formel (Hill-System): C₁₄H₂₁N₃O · 2HCl
• CAS-Nummer: 146986-50-7
• Molekulargewicht: 320.26
• MDL number: MFCD18829832
• UNSPSC Code: 12352200
• NACRES: NA.77
• Assay: ≥95% (HPLC)
• Form: liquid
• Storage condition: OK to freeze, avoid repeated freeze/thaw cycles, protect from light

Eigenschaften

• Produktname: Y-27632, InSolution, ≥95%, 10 mM aqueous solution, Sterile-Filtered, inhibitor of Rho-associated protein kinases
• Quality Segment: 100
• assay: ≥95% (HPLC)
• form: liquid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles, protect from light
• storage temp.: −20°C
• SMILES string: N[C@@H](C1CCC(CC1)C(=O)Nc2ccncc2)C
• InChI: 1S/C14H21N3O/c1-10(15)11-2-4-12(5-3-11)14(18)17-13-6-8-16-9-7-13/h6-12H,2-5,15H2,1H3,(H,16,17,18)/t10-,11?,12?/m1/s1
• InChI key: IYOZTVGMEWJPKR-VOMCLLRMSA-N

Beschreibung

General description

A 10 mM (2 mg/591 µL) sterile-filtered solution of Y-27632 in H₂O.

Ready to use, cell culture-tested, suitable for stem cell applications. Acts as a highly potent, cell-permeable, reversible, and selective inhibitor of Rho-associated protein kinases (K_i = 140 nM for p160ROCK). Also shown to improve survival of human stem cells especially during cell culture passage and manipulation.

Application

InSolution Y-27632, Sterile-Filtered, Cell Culture-Tested, CAS 146986-50-7, is a 10 mM sterile-filtered solution of Y-27632 in H₂O. Acts as an inhibitor of Rho kinases (Ki = 140 nM for p160ROCK).

Biochem/physiol Actions

Primary Target
ROCK

Packaging

Packaged under inert gas

Physical form

A 10 mM (2 mg/591 µL) sterile-filtered solution of Y-27632 (Cat. No. 688000) in H₂O.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 6 months at -20°C.

Other Notes

Li X. et al, et. Al., 2009. Human Reproduction, 24, 580.
Chitaley, K., et al. 2001. Nat. Med.7, 119.
Davies, S.P., et al. 2000. Biochem. J.351, 95.
Narumiya, S., et al. 2000. Methods Enzymol.325, 273.
Hirose, M., et al. 1999. J. Cell Biol.141, 1625.
Maekawa, M., et al. 1999. Science.285, 895.
Uehata, M., et al. 1997. Nature.389, 990.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable