14-925-M

Wee1 Protein, active, 10 µg

Active, N-terminal GST-tagged & C-terminal 6His tagged, recombinant, human Wee1, amino acids 214-end. For use in Kinase Assays.

• UNSPSC Code: 12352202
• NACRES: NA.32
• eCl@ss: 32160405
• Biological source: human
• Recombinant: expressed in baculovirus infected Sf21 cells
• Mol wt: M_w 77 kDa

Eigenschaften

• biological source: human
• Quality Level: 100, 300
• recombinant: expressed in baculovirus infected Sf21 cells
• mol wt: M_w 77 kDa
• manufacturer/tradename: Upstate^®
• technique(s): activity assay: suitable (kinase)
• NCBI accession no.: NM_003390
• UniProt accession no.: P30291
• Gene Information: human ... WEE1(7465)

Beschreibung

General description

Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on ′Tyr-15′. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on ′Tyr-15′ and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.

Product Source: N-terminal GST-tagged and C-terminal 6His tagged, recombinant, human Wee1 amino acids 214-end expressed by baculovirus in Sf21 insect cells.

Biochem/physiol Actions

Protein Target: WEE1

Packaging

Also available in 250μg size as catalog # 14-925M and in bulk as catalog # 14-925-K.

Analysis Note

Routinely evaluated by phosphorylation of peptide LSNLYHQGKFLQTFCGSPLYRRR.

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• pictograms: GHS07
• signalword: Warning
• hcodes: H317
• pcodes: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Lagerklasse: 12 - Non Combustible Liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable