1.25074

Eshmuno^® Q-Harz

MiniChrom Column Eshmuno^® Q, volume 5 mL, prepacked column

• Synonym(e): MiniChrom Column Eshmuno^® Q, 5 ml
• UNSPSC Code: 41115711

Eigenschaften

• ligand: (Trimethylammoniumethyl)
• material: polypropylene housing
• product line: Eshmuno^®
• form: prepacked column
• parameter: ≤8 bar pressure, 1000 cm/hr max. flow rate
• bed L: 100 mm
• bed volume: 5 mL
• column I.D.: 8 mm
• volume: 5 mL
• matrix active group: Polyvinyl Ether
• mean particle size: 85 μm
• capacity: ~150 mg, BSA binding capacity
• application(s): mAb; recombinant protein; vaccine development
• separation technique: anion exchange
• storage temp.: 15-25°C

Beschreibung

General description

8 x 100mm

MiniChrom Pre-packed Columns with Eshmuno^® cation exchange resin is designed for the rapid evaluation of resins for bioprocess development. The results obtained for biomolecule separations are generally comparable to the results achieved using laboratory-scale columns (e.g. 100 x 16 mm ID).

In bioprocess design, the MiniChrom columns can be used to rapidly determine whether a target biomolecule is bound to and can be eluted from the Eshmuno^® resin, as well as determine whether an appropriate target yield may be achieved and whether major contaminants could potentially be removed.

MiniChrom columns are designed for process development, method optimization and parameter screening as well as sample preparation. MiniChrom columns are quality checked for HETP and asymmetry, providing superior reproducibility, excellent scalability, convenient comparison of different chromatography media.

MiniChrom columns are made from biocompatible polypropylene (PP) and polyethylene (PE) and are available in 1 and 5 mL bed volumes. The 8mm diameter columns allow for scale-up from 1 to 5mL column volumes, with a constant internal diameter. These columns are compatible with any chromatographic work station, HPLC system or peristaltic pump.

Features and Benefits

Eshmuno^® Q chromatography resin enables:

• Purification of acidic and neutral proteins and peptides from different sources, including plasma

Superior productivity for downstream processing of biomolecules

• High flow rate versus pressure-flow behavior
• Excellent removal of impurities, such as HCP, leached Protein A, and viruses
• Robust and safe packing procedures
• Strong chemical stability

Legal Information

Eshmuno is a registered trademark of Merck KGaA, Darmstadt, Germany