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05-565

Anti-Nucleolin Antibody, clone 3G4B2

culture supernatant, clone 3G4B2, Upstate®

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UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
3G4B2, monoclonal
Application:
ICC, IP, WB
Citations:
20
Technischer Dienst
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Unterstützung erhalten

biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

culture supernatant

antibody product type

primary antibodies

clone

3G4B2, monoclonal

species reactivity

human, canine, monkey

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

isotype

IgG

NCBI accession no.

NM_005381

UniProt accession no.

P19338

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... NCL(4691)

General description

100kDa
Nucleolin, also known as C23, is the major nucleolar protein of exponentially growing eukaryotic cells. It is a ubiquitous conserved multi-domain phosphoprotein found in all vertebrate species. Nucleolin contains a specific bipartite nuclear localization signal sequence and three different structural domains. Nucleolin is a multifunctional protein involved in most steps of ribosome biogenesis. Nucleolin has been implicated in chromatin structure, rDNA transcription, rRNA maturation, ribosome assembly, cytokinesis, nucleogenesis, cell proliferation and growth, and
nucleo-cytoplasmic transport. Nucleolin also exhibits autodegradation, DNA and RNA helicase activities, and DNA-dependent ATPase activity. Nucleolin activities may be regulated by proteolysis, methylation, ADPribosylation, and phosphorylation by several kinases, including casein kinase II (CK2), p34cdc2, and protein kinase C-ζ.

Immunogen

Nuclear matrix extract from MDCK cells

Application

Anti-Nucleolin Antibody, clone 3G4B2 is a high quality Mouse Monoclonal Antibody for the detection of Nucleolin & has been validated in IP, WB, ICC.

Biochem/physiol Actions

nucleolin

Physical form

mouse culture supernatant containing 0.05% sodium azide prior to the addition of glycerol to 30%

Analysis Note

Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.
routinely evaluated by immunoblot on HeLa nuclear extract

Other Notes

Replaces: MABE466

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Lagerklasse

10 - Combustible liquids

wgk

WGK 1

Analysenzertifikate (COA)

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Verwandter Inhalt

RNA-Binding Protein Immunoprecipitation

All eukaryotic organisms require tight regulation of gene expression for complex processes such as development, differentiation, cell specification, and responses to environmental stimuli. Many genes are regulated post-transcriptionally, in addition to transcriptional mechanisms of gene regulation. RNA-binding proteins (RBPs) are essential for post-transcriptional gene regulation, linking transcription and translation in many processes including transcription, splicing, export, rate of translation and turnover. In all of these events, RBPs coordinate regulation of the amount of protein produced from mRNA transcripts.

Ingrid Morland et al.
Nucleic acids research, 30(22), 4926-4936 (2002-11-16)
The mild phenotype associated with targeted disruption of the mouse OGG1 and NTH1 genes has been attributed to the existence of back-up activities and/or alternative pathways for the removal of oxidised DNA bases. We have characterised two new genes in
Gerhard Dürnberger et al.
Genome biology, 14(7), R81-R81 (2013-08-02)
The interactions between proteins and nucleic acids have a fundamental function in many biological processes, including gene transcription, RNA homeostasis, protein translation and pathogen sensing for innate immunity. While our knowledge of the ensemble of proteins that bind individual mRNAs
J Gotzmann et al.
Electrophoresis, 18(14), 2645-2653 (1998-04-04)
A monoclonal antibody was raised against a salt-extractable fraction of nuclear matrix / intermediate filament scaffolds of polarized MDCK cells. The antibody recognized an approximately 100 kDa protein in total cell lysates and nuclear matrices of various human cells and
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Global Trade Item Number

SKUGTIN
05-56504053252475498