05-1245

Anti-O-GlcNAc Antibody, clone 9D1.E4(10)

clone 9D1.E4(10), from mouse

• Synonym(e): O-GlcNAc transferase p110 subunit, O-GlcNAc transferase subunit p110, O-linked GlcNAc transferase, O-linked N-acetylglucosamine (GlcNAc) transferase ; ; (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase, O-linked N-acetylglucosam
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: 9D1.E4(10), monoclonal
• Species reactivity: human
• Application: WB
• Citations: 6

Eigenschaften

• Quality Segment: 100
• biological source: mouse
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 9D1.E4(10), monoclonal
• species reactivity: human
• technique(s): western blot: suitable
• isotype: IgG1
• NCBI accession no.: NP_858058.1
• UniProt accession no.: O15294
• shipped in: wet ice
• target post-translational modification: glycosylation
• Gene Information: human ... OGT(8473)

Beschreibung

General description

O-linked N-acetylglucosamine (O-GlcNAc), is a member of the O-GlcNAc transferase family. O-GlcNAc is a posttranslational modification characterized by the attachment of N-acetylglucosamine to specific serine/threonine hydroxyl groups. It plays an important role in nutrient sensing, gene expression, and protein degradation. O-GlcNAc is widely expressed in the pancreas and is present in smaller amounts in skeletal muscle, heart, brain and placenta.

Various

Immunogen

SANM-conjugated linear peptide corresponding to human O-GlcNAc.

Application

Detect O-GlcNAc using this Anti-O-GlcNAc Antibody, clone 9D1.E4(10) validated for use in WB.

Research Category
Signaling

Research Sub Category
General Post-translation Modification

Biochem/physiol Actions

This antibody recognizes O-GlcNAc.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal antibody in 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% sodium azide.

Preparation Note

Maintain refrigerated at 2-8°C for up to 12 months.

Analysis Note

Control
HEK293 cell lysate

Evaluated by Western Blot in O-GlcNAc transferease transfected HEK293 cell lysate and O-GlcNAcase transfected transfected HEK293 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected O-GlcNAc on 10 µg of
O-GlcNAc transferease transfected HEK293 cell lysate and O-GlcNAcase transfected transfected HEK293 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable