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HPLC Analysis of Polyphenols in Nero d′Avola Red Wine on Discovery® HS C18 (UV 280 nm)

HPLC Analysis of Polyphenols in Nero d’Avola Red Wine on Discovery® HS C18 (UV 280 nm) application for HPLC

Materials

analytical column

Product No.
Description
Pricing

Discovery® HS C18 HPLC Column

3 μm particle size, L × I.D. 5 cm × 2.1 mm

standard

Product No.
Description
Pricing

Caffeic acid

≥98.0% (HPLC)

Rutin hydrate

≥94% (HPLC), powder

Ethyl gallate

antioxidant, ≥96.0% (HPLC)

Procyanidin B2

analytical standard

Epicatechin solution

certified reference material, 2000 μg/mL in methanol, ampule of 0.5 mL

Isoquercitrin

primary reference standard

(+)-Catechin

analytical standard

Gallic acid

certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

Caftaric acid

analytical standard

CONDITIONS

sample/matrix

Nero d’Avola wine sample filtered prior injection through a 0.45 μm nylon membrane

column

Discovery HS C18, 15 cm x 2.1 mm I.D., 3 μm particles (569255-U)

mobile phase

[A] water, pH 3 with formic acid; [B] acetonitrile, pH 3 with formic acid

flow rate

0.2 mL/min

column temp.

30 °C

detector

UV, 280 nm

injection

5 μL

Description

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany