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Implication of free cholesterol in LC-MS response enhancement.

Bioanalysis (2012-06-02)
Robert MacNeill, Amy Farside, Vishva Acharya, Ryan Stromeyer, Bryan Urbanowicz, Marc Moussallie
ABSTRACT

In the context of matrix effects in bioanalytical LC-MS/MS, a speculative link between free cholesterol, the recoveries of the compound from three common extraction procedures, and response enhancement was qualitatively investigated. Injections on-column of cholesterol both in solution and extracts, in conjunction with post-column infusion of three representative drugs, reveal a direct role in pronounced response enhancement for two out of three analytes, under one set of LC-MS/MS conditions, for the majority of typical plasma extraction procedures, where electrospray-based gaseous ion generation is used. Cholesterol has been shown to have a strong association with LC-MS/MS response enhancement and ideally should be monitored during method development, reinforcing the reasoning behind minimizing SPE elution volumes and avoiding less selective means of sample preparation.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Rizatriptan benzoate salt, ≥98% (HPLC)