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  • Endogenous Biosynthesis of S-Nitrosoglutathione From Nitro-Fatty Acids in Plants.

Endogenous Biosynthesis of S-Nitrosoglutathione From Nitro-Fatty Acids in Plants.

Frontiers in plant science (2020-07-28)
Capilla Mata-Pérez, María N Padilla, Beatriz Sánchez-Calvo, Juan C Begara-Morales, Raquel Valderrama, Mounira Chaki, Lorena Aranda-Caño, David Moreno-González, Antonio Molina-Díaz, Juan B Barroso
ABSTRACT

Nitro-fatty acids (NO2-FAs) are novel molecules resulting from the interaction of unsaturated fatty acids and nitric oxide (NO) or NO-related molecules. In plants, it has recently been described that NO2-FAs trigger an antioxidant and a defence response against stressful situations. Among the properties of NO2-FAs highlight the ability to release NO therefore modulating specific protein targets through post-translational modifications (NO-PTMs). Thus, based on the capacity of NO2-FAs to act as physiological NO donors and using high-accuracy mass-spectrometric approaches, herein, we show that endogenous nitro-linolenic acid (NO2-Ln) can modulate S-nitrosoglutathione (GSNO) biosynthesis in Arabidopsis. The incubation of NO2-Ln with GSH was analyzed by LC-MS/MS and the in vitro synthesis of GSNO was noted. The in vivo confirmation of this behavior was carried out by incubating Arabidopsis plants with 15N-labeled NO2-Ln throughout the roots, and 15N-labeled GSNO (GS15NO) was detected in the leaves. With the aim to go in depth in the relation of NO2-FA and GSNO in plants, Arabidopsis alkenal reductase mutants (aer mutants) which modulate NO2-FAs levels were used. Our results constitute the first evidence of the modulation of a key NO biological reservoir in plants (GSNO) by these novel NO2-FAs, increasing knowledge about S-nitrosothiols and GSNO-signaling pathways in plants.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Sodium nitrite-15N, 98 atom % 15N, 95% (CP)
Sigma-Aldrich
L-Glutathione reduced, ≥98.0%
Sigma-Aldrich
S-Nitrosoglutathione, ≥97%
Supelco
F.A.M.E. Mix, C4-C24, certified reference material,  wt. % (varied)