581424-U
Ascentis® C8 (5 µm) HPLC Columns
L × I.D. 15 cm × 4.6 mm, HPLC Column
Synonym(s):
C8 Reversed-Phase Column
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About This Item
UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
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product name
Ascentis® C8 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm
material
stainless steel column
Agency
suitable for USP L7
product line
Ascentis®
feature
endcapped
manufacturer/tradename
Ascentis®
packaging
1 ea of
extent of labeling
15% Carbon loading
parameter
≤70 °C temp. range
400 bar pressure (5801 psi)
technique(s)
HPLC: suitable
LC/MS: suitable
L × I.D.
15 cm × 4.6 mm
surface area
450 m2/g
surface coverage
4.0 μmol/m2
impurities
<5 ppm metals
matrix
fully porous particle
silica gel high purity, spherical
matrix active group
C8 (octyl) phase
particle size
5 μm
pore size
100 Å
operating pH range
2-8
application(s)
food and beverages
separation technique
reversed phase
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General description
The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.
The Ascentis C8 is a highly reproducible column with excellent selectivity towards polar compounds. The phase excells in highly aqueous mobile phases as well as high organic mobile phases.
The Ascentis C8 is a highly reproducible column with excellent selectivity towards polar compounds. The phase excells in highly aqueous mobile phases as well as high organic mobile phases.
Application
- Analysis of 27 beta-Blockers and Metabolites in Milk Powder by High Performance Liquid Chromatography Coupled to Quadrupole Orbitrap High-Resolution Mass Spectrometry.: This study utilizes the Ascentis® C8 HPLC Column to analyze beta-blockers and their metabolites in milk powder, highlighting the column′s capacity for sensitive and precise quantification in complex matrices (Cheng et al., 2019).
- Recent trends in ultra-fast HPLC: new generation superficially porous silica columns.: A review of advancements in HPLC technology, including the use of Ascentis® C8 columns, which enhance the speed and efficiency of chemical separations (Ali et al., 2012).
- Superficially porous particles columns for super fast HPLC separations.: Discusses the benefits of using Ascentis® C8 HPLC Columns for achieving faster and more efficient separations in HPLC, underlining their utility in high-throughput settings (Ali et al., 2012).
- Gradient RP-HPLC method for the determination of potential impurities in atazanavir sulfate.: Demonstrates the effectiveness of the Ascentis® C8 HPLC Column in a robust method for identifying impurities in pharmaceutical compounds, ensuring product safety and compliance (Chitturi et al., 2011).
- Development and validation of an HPLC-MS/MS method to determine clopidogrel in human plasma. Use of incurred samples to test back-conversion.: Illustrates the application of Ascentis® C8 HPLC Column in a validated method for the analysis of clopidogrel, improving therapeutic drug monitoring and patient care (Silvestro et al., 2010).
Features and Benefits
- Superior retention for hydrophobic molecules
- Excellent peak shape
- Low bleed LC-MS separations
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
guard cartridge
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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