ZRB1374

Anti-Glypican-1 Antibody, clone 1J4, ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): GPC1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit (recombinant)
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1J4, monoclonal; recombinant monoclonal
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 61.68 kDa; observed mol wt ~90 kDa
• species reactivity: human
• species reactivity (predicted by homology): monkey
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• UniProt accession no.: P35052
• greener alternative category: Aligned,
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... GPC1(2817) ; rhesus monkey ... Gpc1(698326)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone 1J4 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Glypican-1. It targets an epitope within 24 amino acids from the C-terminal half.

Immunogen

KLH-conjugated liner peptide corresponding to 24 amino acids from the C-terminal half of human Glypican-1, isoform 1.

Application

Anti-Glypican-1, clone 1J4 ZooMAb, Cat. No. ZRB1374, a recombinant Rabbit monoclonal antibody that targets Glypican-1 and is tested in Affinity Binding, Immunofluorescence, and Immunohistochemistry, and Western Blotting.

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Glypican-1 in MDA-MB-231 cell lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Glypican-1 in human pancreatic cancer tissue sections.

Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected Glypican-1 in human pancreatic cancer tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound Glypican-1 peptide with a KD of 4.3 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Glypican-1 (UniProt: P35052; also known as GPC1) is encoded y the GPC1 gene (Gene ID: 2817) in human. Glypican-1 is a cell-surface glycoprotein that attaches to the plasma membrane via via glycosylphosphatidylinositol (GPI) anchors. It is synthesized with a signal peptide (aa 1-23) and a propeptide (aa 531-538) that are subsequently cleaved off to produce mature protein. Glypican-1 undergoes N- and O-glycosylations. N-glycosylation is mainly of the complex type containing sialic acid whereas O-glycosylation is with heparan sulfate Heparan sulfate chains are cleaved either by heparinase or are degraded by deaminative process that uses nitric oxide released from the S-nitrosylated cysteines. Glypican-1 is shown to be essential for proper skeletal muscle differentiation. It can act as a catalyst in increasing the rate of conversion of prion protein PRPN(C) to PRNP(Sc) via associating with both forms of PRPN, targeting them to lipid rafts and facilitating their interaction. It can also sequester FGF2 in lipid rafts and blocks its binding to FGF receptors. Glypican-1 is specifically enriched on cancer-cell-derived exosomes and levels of glypican-1 positive exosomes correlate with tumor burden and the survival of pre- and post-surgical patients. It is also over-expressed in human gliomas and in pancreatic cancer cells. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Melo, SA et al. 2015. Nature. 523(7559):177-82; Kleeff, J., et al. 1998. J. Clin. Invest. 102(9), 1662-1673).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2