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MHA00MRS2

Millipore

De Man, Rogosa and Sharpe Broth

capacity 2 mL, For final product testing or in-process testing, for lactic acid bacteria, input: beverage(s)

Synonym(s):

MRS broth

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About This Item

UNSPSC Code:
41106225
eCl@ss:
32160801
NACRES:
NA.74

Quality Level

sterility

sterile

form

liquid

shelf life

12 mo.

feature

ready-to-use

packaging

pack of 50 tests

capacity

2 mL

filling volume

2 mL

color

clear red-brown

input

sample type beverage(s)
sample type sport drink
sample type cider
sample type soft drink
sample type beer
sample type wine
sample type cosmetics
sample type food(s)
sample type bottled water

pH

6.5±0.2 (25.0 °C)

application(s)

clinical
cosmetics
environmental
food and beverages
industrial qc
microbiology
water monitoring

compatibility

for use with MF-method
for use with Microfil®
for use with MicropreSure®
for use with Milliflex®

shipped in

wet ice

storage temp.

2-8°C

suitability

Lactobacillus brevis
Lactobacillus fermenti
lactic acid bacteria

General description

MRS Broth is used for the enrichment, cultivation and isolation of all species of Lactobacillus. The MRS media formulation was developed by de Man, Rogosa and Sharpe {23} to replace the tomato juice medium and the meat extract tomato juice medium. It is a medium supporting good growth of lactobacilli in general, even those strains which have shown poor growth in existing media, like strains of L. brevis and L. fermenti. The MRS culture media contain polysorbate (Tween 80), acetate, magnesium and manganese which are known to act as special growth factors for Lactobacilli as well as a rich nutrient base. As these media show a very low degree of selectivity Pediococcus and Leuconostoc species as well as other secondary bacteria may grow on them. Most of the accompanying microflora can be inhibited by thallium acetate, sorbic acid, acetic acid, sodium nitrite, cycloheximide and polymyxin. Lactobacillus species appear as small, white, creamy colonies. Saccharomyces species appear as large, white, creamy colonies.{24}

Application

MRS Broth is used for final product testing or in-process testing of beverages for the contamination by lactobacilli, L. brevis and L. fermenti in Beer, Bottled Water, Cider, Soft Drink and Sport Drink. This media finds application in Clinical, Cosmetics and Food and Beverage industry.

Culture Medium

MRS

Analysis Note

Lactobacillus species appear as small, white, creamy colonies. Saccharomyces species appear as large, white, creamy colonies
QC organisms
L. casei (ATCC 393)

Legal Information

MICROFIL is a registered trademark of Merck KGaA, Darmstadt, Germany
MICROPRESURE is a registered trademark of Merck KGaA, Darmstadt, Germany
MILLIFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williams and Wilkins, Baltimore
MacFaddin JF
The Journal of Applied Bacteriology, 23(1), 130-135 (1985)
A medium for the cultivation of lactobacilli
De Man, JC and Rogosa, d M and Sharpe, M Elisabeth
The Journal of Applied Bacteriology, 23(1), 130-135 (1960)

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