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MAB88918

Sigma-Aldrich

Anti-Laminin Antibody, clone 2G6/A2

clone 2G6/A2, Chemicon®, from mouse

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

2G6/A2, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... LAMA1(284217)

Specificity

The antibody reacts with human laminin both in ELISA and on cells and frozen sections. It is able to precipitate laminin from the culture medium. The antibody does not inhibit neurite outgrowth from neurons induced by laminin as well as the binding of heparin to laminin. It does not react with laminin from rat and mouse.The binding of the antibody to human laminin is not inhibited by the laminin-pentapeptide Tyr-Ile-Gly-Ser-Arg (Iwanmoto, 1987).The staining of laminin was tested on a number of materials including human lung fibroblasts (IMR 90) and human skeletal muscle tissue.

Immunogen

Pepsin fragments of human laminin from placenta.

Application

Immunocytochemistry: (5-20 μg/mL)

ELISA: (5-20 μg/mL)

Immunohistochemistry: (5-20 μg/mL) Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
ECM Proteins
This Anti-Laminin Antibody, clone 2G6/A2 is validated for use in ELISA, IP, IC, IH for the detection of Laminin.

Physical form

Format: Purified
Purified immunoglobulin. Liquid in 0.01 M Phosphate buffer, 0.15 M NaCl, pH 7.1, sterile filtered.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 12 months.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Tomoko Nagase et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 238(5), 1118-1130 (2009-04-23)
In routine culture, human embryonic stem (hES) cells are maintained on either feeder cells or special culture substrates such as Matrigel. However, to expand hES cells for clinical applications, it is desirable to minimize animal-derived materials in the culture for

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